Application | Comment | Organism |
---|---|---|
synthesis | production of enzyme using a continuous lactose induction strategy. The lactose concentration in the feed medium affects directly the expression of the target protein. The use of 50 g/L in the feed medium results in an expression level of above 30%, and the maximum final enzyme concentration of 16200 U/l. The acetate concentration remains at a low level in the fed-batch phase, less than 0.5 g/l | Hyperthermus butylicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Hyperthermus butylicus |
expression in Escherichia coli | Hyperthermus butylicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyperthermus butylicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribose 5-phosphate | - |
Hyperthermus butylicus | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-Deoxyribose-5-phosphate aldolase | - |
Hyperthermus butylicus |
DERA | - |
Hyperthermus butylicus |
Organism | Comment | Expression |
---|---|---|
Hyperthermus butylicus | lactose can induce DERA formation efficiently as isopropyl-beta-D-thiogalactoside. The enzyme concentration increases with the lactose content and reaches a plateau at a concentration of 2.0 g/l | up |