Application | Comment | Organism |
---|---|---|
biotechnology | efficient process for the production of thermophilic DERA designed from the point of view of recombinant enzyme concentration and productivity, which is economically and technically viable and can be used as a guide for production of other synthetically useful enzymes | Hyperthermus butylicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) from pET303-DERA008 in both batch and fed-batch cultures, under the control of the IPTG-inducible T7 promoter | Hyperthermus butylicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyperthermus butylicus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
2-Deoxyribose-5-phosphate aldolase | - |
Hyperthermus butylicus |
DERA | - |
Hyperthermus butylicus |
Organism | Comment | Expression |
---|---|---|
Hyperthermus butylicus | when the concentration of acetate is over 1.0 g/l, DERA expression level decreases | down |
Hyperthermus butylicus | 2 mmol IPTG induces the expression of DERA. As the induction proceeds, the target protein expression level first increases and then decreases, and DERA concentration improves and reaches a plateau after 6 h of induction. DERA concentration increases with the biomass growth and peaks at the end of the culture period at 5.12 g/l with total volumetric productivity of 0.256 gl-1h-1, which are over 10 and 5fold higher than conventional batch culture, respectively | up |
General Information | Comment | Organism |
---|---|---|
physiological function | DERA catalyzes a sequential aldol reaction that is useful to synthesize a key fragment of atorvastatin | Hyperthermus butylicus |