Application | Comment | Organism |
---|---|---|
synthesis | mutant D-2-deoxyribose-5-phosphate aldolase Ser238Asp is used to prepare beta-hydroxy-delta lactol synthons and tert-butyl [(4R,6R)-6-aminoethyl-2,2-dimethyl-1,3-dioxn-4-yl]acetate, a key intermediate for atorvastatin synthesis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S238D | mutant enzyme shows great improvement in catalytic activity towards sequential aldol reactions, mutant enzyme has wider specificity than wild-type enzyme, mutant enzyme shows activity with 3-azidopropionaldehyde and acetaldehyde, wild-type enzyme shows no activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-azidopropionaldehyde + acetaldehyde | no activity with wild-type enzyme, mutant enzyme S238D gives 35% yield of the sequential aldol condensation product | Escherichia coli | (4R,6R)-4-hydroxy-6-(2-triaz-2-en-1-ylethyl)tetrahydro-2H-pyran-2-one | - |
? | |
3-chloropropionaldehyde + acetaldehyde | wild type enzyme gives 25% yield of the sequential aldol condensation product, mutant enzyme S238D gives 43% yield of the sequential aldol condensation product | Escherichia coli | (4R,6S)-6-(2-chloroethyl)-4-hydroxytetrahydro-2H-pyran-2-one | - |
? | |
additional information | no activity with 3-nitropropionaldehyde + acetaldehyde, wild-type enzyme and mutant enzyme S238D | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-2-deoxyribose-5-phosphate aldolase | - |
Escherichia coli |
DERA | - |
Escherichia coli |