Literature summary for 4.1.2.38 extracted from

Schmidt, T.; Zavrel, M.; Spiess, A.; Ansorge-Schumacher, M.B.
Biochemical peculiarities of benzaldehyde lyase from Pseudomonas fluorescens Biovar I in the dependency on pH and cosolvent concentration (2009), Bioorg. Chem., 37, 84-89.

Search for more literature for 4.1.2.38

Activating Compound

Activating Compound	Comment	Organism	Structure
dimethyl sulfoxide	highest activity between 20% and 30% dimethyl sulfoxide (v/v) with a peak at 25%. Records of pH-activity profiles at different dimethyl sulfoxide concentrations and constant ionic strength (100 mM) reveals a strong influence of the cosolvent on the activity of BAL, and indicates a cross effect on the optimal pH with a shift of profiles to the alkaline milieu. Correlation between the dependency on pH and dimethyl sulfoxide concentration is due to interaction of dimethyl sulfoxide with the amino acid side chain Glu50 in the catalytic site of the biocatalyst	Pseudomonas fluorescens
additional information	synthetic BAL activity in KH2PO4 buffer (pH 8.5, 30% v/v dimethyl sulfoxide) reveals that the ionic strength of the buffer system has a very strong reciprocal effect on the activity of BAL, i.e. in a range between 25 mM and 500 mM, highest initial rates are observed at lowest ionic strength, and activity decreases with increasing ionic strength	Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli from SG13009prep4 (pBAL-His6)	Pseudomonas fluorescens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	biovar I	-

Storage Stability

Storage Stability	Organism
-20°C	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,5-dimethoxy-benzaldehyde + 3,5-dimethoxy-benzaldehyde	-	Pseudomonas fluorescens	(R)-3,3',5,5'-tetramethoxy-benzoin	-	?

Synonyms

Synonyms	Comment	Organism
BAL	-	Pseudomonas fluorescens
benzaldehyde lyase	-	Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	increase of the optimal pH of BAL with increasing concentrations of dimethyl sulfoxide	Pseudomonas fluorescens

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)