Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli cells | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for second binding substrate, mechanistic model | Pseudomonas fluorescens | |
0.007 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for second binding substrate, mechanistic model | Pseudomonas fluorescens | |
0.01 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for first binding substrate, simplified model | Pseudomonas fluorescens | |
0.012 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for first binding substrate, simplified model | Pseudomonas fluorescens | |
0.042 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for second binding substrate, simplified model | Pseudomonas fluorescens | |
0.047 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for second binding substrate, simplified model | Pseudomonas fluorescens | |
0.05 | - |
3,5-dimethoxy-benzaldehyde | Michaelis constant for first binding substrate, mechanistic model | Pseudomonas fluorescens | |
0.975 | - |
(R)-3,3',5,5'-tetramethoxy-benzoin | Michaelis constant for product, mechanistic model | Pseudomonas fluorescens | |
1.205 | - |
(R)-3,3',5,5'-tetramethoxy-benzoin | Michaelis constant for product, mechanistic model | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58919 | - |
4 * 58919 | Pseudomonas fluorescens |
58920 | - |
monomer, calculated from sequence | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-1,2-diphenylethanone | Pseudomonas fluorescens | - |
benzaldehyde | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni2+-affinity chromatography | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-1,2-diphenylethanone | - |
Pseudomonas fluorescens | benzaldehyde | - |
r | |
3,5-dimethoxy-benzaldehyde | - |
Pseudomonas fluorescens | (R)-3,3',5,5'-tetramethoxy-benzoin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 58919 | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
BAL | - |
Pseudomonas fluorescens |
benzaldehyde lyase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45.44 | - |
3,5-dimethoxy-benzaldehyde | simplified model | Pseudomonas fluorescens | |
46.12 | - |
3,5-dimethoxy-benzaldehyde | mechanistic model | Pseudomonas fluorescens | |
46.15 | - |
3,5-dimethoxy-benzaldehyde | mechanistic model | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity assay | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas fluorescens |