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Literature summary for 4.1.2.38 extracted from
- Reaction engineering of benzaldehyde lyase from Pseudomonas fluorescens catalyzing enantioselective C-C bond formation (2006), Org. Proc. Res. Dev., 10, 1172-1177.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Benzoin | in TEA buffer (35 mM, pH 8) containing 0.35 mM thiamine diphosphate, 0.35 mM MgSO4, and 30% (v/v) DMSO, at 20°C | Pseudomonas fluorescens |  |
| 2.2 | - |
benzaldehyde | in TEA buffer (35 mM, pH 8) containing 0.35 mM thiamine diphosphate, 0.35 mM MgSO4, and 30% (v/v) DMSO, at 20°C | Pseudomonas fluorescens | |
| 6 | - |
acetaldehyde | in TEA buffer (35 mM, pH 8) containing 0.35 mM thiamine diphosphate, 0.35 mM MgSO4, and 30% (v/v) DMSO, at 20°C | Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 0.5 mM, substantial for the stability of BAL | Pseudomonas fluorescens | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | BAL is stabilized significantly in the presence of 30% (v/v) DMSO | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-benzoin | - |
Pseudomonas fluorescens | benzaldehyde + benzaldehyde | - |
r | |
| 3-chlorobenzaldehyde + acetaldehyde | - |
Pseudomonas fluorescens | (R)-(3-chlorophenyl)-2-hydroxy-1-propanone | - |
r | |
| benzaldehyde + acetaldehyde | - |
Pseudomonas fluorescens | (R)-2-hydroxy-1-phenylpropanone | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BAL | - |
Pseudomonas fluorescens |
| benzaldehyde lyase | - |
Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
highest carboligation activity | Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
maximum of enzyme stability is achieved at pH 7 | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | 0.5 mM, substantial for the stability of BAL | Pseudomonas fluorescens | |
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