BRENDA - Enzyme Database
show all sequences of 4.1.2.37

alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization

Caruso, C.S.; de Fatima Travensolo, R.; de Campus Bicudo, R.; de Macedo Lemos, E.G.; Ulian de Araujo, A.P.; Carrilho, E.; Microb. Pathog. 47, 118-127 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Xylella fastidiosa
Crystallization (Commentary)
Crystallization
Organism
circular dichroism study. The spectral deconvolution in pH 7.8 estimates 35.5% helical elements, 11.1% beta structures, and 53.4% other structures
Xylella fastidiosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29100
-
2 * 30400, GelChip-CE, 2 * 29100, calculated, recombinant protein including His-tag
Xylella fastidiosa
30400
-
2 * 30400, GelChip-CE, 2 * 29100, calculated, recombinant protein including His-tag
Xylella fastidiosa
61900
-
gel filtration
Xylella fastidiosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xylella fastidiosa
Q9PHH3
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Xylella fastidiosa
Subunits
Subunits
Commentary
Organism
dimer
2 * 30400, GelChip-CE, 2 * 29100, calculated, recombinant protein including His-tag
Xylella fastidiosa
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
FAD-independent
Xylella fastidiosa
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Xylella fastidiosa
isoelectric focusing
-
4.6
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Xylella fastidiosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
FAD-independent
Xylella fastidiosa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
circular dichroism study. The spectral deconvolution in pH 7.8 estimates 35.5% helical elements, 11.1% beta structures, and 53.4% other structures
Xylella fastidiosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29100
-
2 * 30400, GelChip-CE, 2 * 29100, calculated, recombinant protein including His-tag
Xylella fastidiosa
30400
-
2 * 30400, GelChip-CE, 2 * 29100, calculated, recombinant protein including His-tag
Xylella fastidiosa
61900
-
gel filtration
Xylella fastidiosa
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Xylella fastidiosa
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30400, GelChip-CE, 2 * 29100, calculated, recombinant protein including His-tag
Xylella fastidiosa
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Xylella fastidiosa
isoelectric focusing
-
4.6
Other publictions for EC 4.1.2.37
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709831
Caruso
alpha-Hydroxynitrile lyase pro ...
Xylella fastidiosa
Microb. Pathog.
47
118-127
2009
-
-
1
1
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-