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Literature summary for 4.1.2.32 extracted from
- Non-oxidative demethylation of trimethylamine N-oxide by Pseudomonas aminovorans (1979), FEBS Lett., 18, 297-300.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is unstable when purified more than about 5-fold | Aminobacter aminovorans |
| exposure of the enzyme for 30 min at 0°C to buffers of pH values in the region 4 to 9 does not result in loss of activity followed by adjustment of the pH 6.0 | Aminobacter aminovorans |
| trimethylamine-grown Pseudomonas aminovorans enzyme is more labile at 40°C than enzyme from methylamine-grown bacteria in French press extracts | Aminobacter aminovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | active at 0.001 mM | Aminobacter aminovorans |  |
| Mercurials | active at 0.001 mM | Aminobacter aminovorans | |
| Triethylamine N-oxide | - |
Aminobacter aminovorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
Trimethylamine N-oxide | - |
Aminobacter aminovorans | |
| 140 | - |
Benzyldimethylamine N-oxide | - |
Aminobacter aminovorans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aminobacter aminovorans | enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aminobacter aminovorans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Aminobacter aminovorans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trimethylamine N-oxide = dimethylamine + formaldehyde | non-oxidative, non-hydrolytic cleavage | Aminobacter aminovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyldimethylamine N-oxide | 50% of the activity with trimethylamine N-oxide | Aminobacter aminovorans | ? | - |
? | |
| additional information | enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium | Aminobacter aminovorans | ? | - |
? | |
| Trimethylamine N-oxide | - |
Aminobacter aminovorans | Dimethylamine + formaldehyde | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Aminobacter aminovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aminobacter aminovorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | pH 5.0: about 75% of maximal activity, pH 7.5: about 75% of maximal activity | Aminobacter aminovorans |
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Release 2023.1 (January 2023)
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