Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.27 extracted from

  • Reina, E.; Camacho, L.; Casas, J.; Van Veldhoven, P.P.; Fabrias, G.
    Determination of sphingosine-1-phosphate lyase activity by gas chromatography coupled to electron impact mass spectrometry (2012), Chem. Phys. Lipids, 165, 225-231.
    View publication on PubMed
Search for more literature for 4.1.2.27

Application

Application Comment Organism
analysis easy and sensitive procedure to determine sphingosine-1-phosphate lyase activity. The assay uses C17-sphinganine-1-phosphate as substrate and the aldehyde product, pentadecanal, is quantified as its pentafluorobenzyloxime derivative by GC/MS. Derivatization of pentadecanal is performed as a one-step reaction, and the oxime product is directly injected for GC/MS analysis without any further purification. Limit of detection is 281 fmol. The assay is linear with both protein concentration and incubation time up to 20 microg and 40 min, respectively Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Deoxypyridoxine phosphate
-
 Mus musculus
FTY720 produces an 82% inhibition at equimolar concentration with C17-sphinganine-1-phosphate Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 C17-sphinganine-1-phosphate pH 7.4, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryonic fibroblast
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C17-sphinganine-1-phosphate
-
 Mus musculus phosphoethanolamine + pentadecanal
-
 ?
sphinganine 1-phosphate
-
 Mus musculus phosphoethanolamine + palmitaldehyde
-
 ?