Literature summary for 4.1.2.27 extracted from

Bandhuvula, P.; Li, Z.; Bittman, R.; Saba, J.D.
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate (2009), Biochem. Biophys. Res. Commun., 380, 366-370.

Search for more literature for 4.1.2.27

Application

Application	Comment	Organism
analysis	use of BODIPY-labeled sphinganine substrate, allows fluorescent product detection by HPLC. The reaction is linear over a 30 min time period	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in HEK-293 cell	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled sphinganine 1-phosphate	37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled sphinganine 1-phosphate	i.e. BODIPY-label, allows fluorescent product detection by HPLC	Homo sapiens	phosphoethanolamine + 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled palmitaldehyde	major aldehyde product is confirmed by reaction with 2,4-dinitrophenylhydrazine	?

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Release 2023.1 (January 2023)