Cloned (Comment) | Organism |
---|---|
the plasmids pRS415, pRS416 and pESC are used | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C344A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
C65L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
C65L/K67L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
C65L/K67L/S70L/N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
DELTA1-16 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
DELTA1-31 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
DELTA1-31/C65L/K67L/S70L/N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
DELTA1-54 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
DELTA1-57 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
DELTA1-81 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
H268A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
H340A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
K380A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
K386A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
K67L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
K67L/S70L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
N6A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
S70L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
S70L/N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
Y554A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
Y554F | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Saccharomyces cerevisiae | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine 1-phosphate | Saccharomyces cerevisiae | - |
phosphoethanolamine + palmitaldehyde | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q05567 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
FLAG-tagged purification is performed | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine 1-phosphate | - |
Saccharomyces cerevisiae | phosphoethanolamine + palmitaldehyde | - |
ir |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Dpl1p | - |
Saccharomyces cerevisiae |
sphinganine-1-phosphate aldolase | - |
Saccharomyces cerevisiae |
sphinganine-1-phosphate lyase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Saccharomyces cerevisiae |