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Literature summary for 4.1.2.27 extracted from

  • Mukhopadhyay, D.; Howell, K.S.; Riezman, H.; Capitani, G.
    Identifying key residues of sphinganine-1-phosphate lyase for function in vivo and in vitro (2008), J. Biol. Chem., 283, 20159-20169.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the plasmids pRS415, pRS416 and pESC are used Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C344A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
C65L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
C65L/K67L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
C65L/K67L/S70L/N71L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
DELTA1-16 mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
DELTA1-31 mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
DELTA1-31/C65L/K67L/S70L/N71L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
DELTA1-54 mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
DELTA1-57 mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
DELTA1-81 mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
H268A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
H340A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
K380A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
K386A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
K67L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
K67L/S70L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
N6A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
N71L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
S70L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
S70L/N71L mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
Y554A mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae
Y554F mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
Saccharomyces cerevisiae 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphinganine 1-phosphate Saccharomyces cerevisiae
-
phosphoethanolamine + palmitaldehyde
-
ir

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q05567
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
FLAG-tagged purification is performed Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine 1-phosphate
-
Saccharomyces cerevisiae phosphoethanolamine + palmitaldehyde
-
ir

Subunits

Subunits Comment Organism
oligomer
-
Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Dpl1p
-
Saccharomyces cerevisiae
sphinganine-1-phosphate aldolase
-
Saccharomyces cerevisiae
sphinganine-1-phosphate lyase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Saccharomyces cerevisiae