Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.27 extracted from

  • Van Veldhoven, P.P.
    Sphingosine-1-phosphate lyase (2000), Methods Enzymol., 311, 244-254.
    View publication on PubMed
Search for more literature for 4.1.2.27

Inhibitors

Inhibitors Comment Organism Structure
2-vinyl sphinganine phosphate 50% inhibition at 0.0024 mM Rattus norvegicus
4-chloromercuribenzoate alkylates a cysteine residue Mammalia
Ca2+ potent inhibitor Mammalia
cyanide inhibits Mammalia
deoxypyridoxin phosphate inhibits Mammalia
iodoacetamide alkylates a cysteine residue Mammalia
N,N-dimethyldodecylamine N-oxide 50% inhibition at 0.05 mM Mammalia
N-ethylmaleimide alkylates a cysteine residue Mammalia
Semicarbazide inhibits Mammalia
sphinganine 1-phosphonate competitive inhibitor Rattus norvegicus
sphinganine phosphate 2D,3L-isomer is a competitive inhibitor, but 2L,3L-isomer does not influence the enzyme Tetrahymena pyriformis
Zn2+ potent inhibitor Mammalia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009 0.016 sphinganine 1-phosphate
-
 Rattus norvegicus
0.11
-
 sphinganine 1-phosphate
-
 Tetrahymena pyriformis

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum catalytic site faces the cytosol Mammalia 5783
-
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000 65000 SDS-PAGE Mus musculus
61000 65000 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mammalia acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mammalia
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-
Tetrahymena pyriformis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde mechanism Mammalia
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
additional information no activity in platelets Mammalia
-

Storage Stability

Storage Stability Organism
-80°C, activity is well preserved in tissues or cells Mammalia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine Mammalia ?
-
 ?
additional information acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine Mus musculus ?
-
 ?
additional information acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine Homo sapiens ?
-
 ?
sphinganine 1-phosphate
-
 Rattus norvegicus phosphoethanolamine + palmitaldehyde
-
 ?
sphinganine 1-phosphate
-
 Tetrahymena pyriformis phosphoethanolamine + palmitaldehyde
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mammalia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 sphinganine 1-phosphonate
-
 Rattus norvegicus
0.0097
-
 sphinganine phosphate 2D,3L-isomer Tetrahymena pyriformis