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Literature summary for 4.1.2.25 extracted from

  • Wang, Y.; Scherperel, G.; Roberts, K.D.; Jones, A.D.; Reid, G.E.; Yan, H.
    A point mutation converts dihydroneopterin aldolase to a cofactor-independent oxygenase (2006), J. Am. Chem. Soc., 128, 13216-13223.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y53F the mutation converts the enzyme to a cofactor-independent oxygenase, which generates mainly 7,8-dihydroxanthopterin Escherichia coli
Y54F the mutation converts the enzyme to a cofactor-independent oxygenase, which generates mainly 7,8-dihydroxanthopterin Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Bio-Gel A-0.5m gel filtration Escherichia coli
Ni-NTA column chromatography and Bio-Gel A-0.5m gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin
-
Staphylococcus aureus 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
?
7,8-dihydroneopterin
-
Escherichia coli 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
?

Synonyms

Synonyms Comment Organism
DHNA
-
Staphylococcus aureus
DHNA
-
Escherichia coli