General Stability | Organism |
---|---|
2-thioethanol stabilizes during purification | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
2-Keto-3-deoxy-L-arabonate | - |
Pseudomonas sp. | |
2.9 | - |
2-Keto-3-deoxy-D-fuconate | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required | Pseudomonas sp. | |
Co2+ | 95% of the activation with Mn2+ | Pseudomonas sp. | |
Mg2+ | divalent cation required | Pseudomonas sp. | |
Mg2+ | 54% of the activity with Mn2+ | Pseudomonas sp. | |
Mn2+ | divalent cation required | Pseudomonas sp. | |
Mn2+ | most effective | Pseudomonas sp. | |
Ni2+ | divalent cation required | Pseudomonas sp. | |
Ni2+ | 45% of the activity with Mn2+ | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas sp. | enzyme functions in the metabolism of L-arabinose and D-fucose | ? | - |
? | |
additional information | Pseudomonas sp. MSU-1 | enzyme functions in the metabolism of L-arabinose and D-fucose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. MSU-1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.63 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M bicine, 0.14 mM 2-thioethanol, 3 weeks, 70% loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Keto-3-deoxy-D-fuconate | - |
Pseudomonas sp. | Pyruvate + D-lactaldehyde | - |
? | |
2-Keto-3-deoxy-D-fuconate | - |
Pseudomonas sp. MSU-1 | Pyruvate + D-lactaldehyde | - |
? | |
2-Keto-3-deoxy-L-arabonate | - |
Pseudomonas sp. | Pyruvate + glycolaldehyde | - |
? | |
2-Keto-3-deoxy-L-arabonate | - |
Pseudomonas sp. MSU-1 | Pyruvate + glycolaldehyde | - |
? | |
additional information | enzyme functions in the metabolism of L-arabinose and D-fucose | Pseudomonas sp. | ? | - |
? | |
additional information | enzyme functions in the metabolism of L-arabinose and D-fucose | Pseudomonas sp. MSU-1 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.3 | - |
Pseudomonas sp. |