BRENDA - Enzyme Database
show all sequences of 4.1.2.14

Extended substrate range of thiamine diphosphate-dependent MenD enzyme by coupling of two C-C-bonding reactions

Schapfl, M.; Baier, S.; Fries, A.; Ferlaino, S.; Waltzer, S.; Mueller, M.; Sprenger, G.A.; Appl. Microbiol. Biotechnol. 102, 8359-8372 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + D-glyceraldehyde 3-phosphate
Escherichia coli
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0A955
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + D-glyceraldehyde 3-phosphate
-
746867
Escherichia coli
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
-
?
pyruvate + glyoxylate
-
746867
Escherichia coli
(4S)-4-hydroxy-2-oxoglutarate
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + D-glyceraldehyde 3-phosphate
Escherichia coli
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + D-glyceraldehyde 3-phosphate
-
746867
Escherichia coli
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
-
?
pyruvate + glyoxylate
-
746867
Escherichia coli
(4S)-4-hydroxy-2-oxoglutarate
-
-
-
r
Other publictions for EC 4.1.2.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746867
Schapfl
Extended substrate range of t ...
Escherichia coli
Appl. Microbiol. Biotechnol.
102
8359-8372
2018
-
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1
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2
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-
747152
Zaitsev
Insights into the substrate s ...
Saccharolobus solfataricus
Biochemistry
57
3797-3806
2018
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1
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1
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4
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748387
Seo
Structures of Zymomonas 2-ket ...
Zymomonas mobilis subsp. molbilis, Zymomonas mobilis subsp. molbilis ATCC 31821
J. Microbiol. Biotechnol.
28
1339-1345
2018
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1
1
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5
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2
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1
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2
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748258
Morita
Heterologous expression of ba ...
Escherichia coli
J. Biosci. Bioeng.
124
263-270
2017
-
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1
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1
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3
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1
1
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748531
Nishiyama
Identification of 2-keto-3-de ...
Flavobacterium sp. UMI-01
Mar. Drugs
15
pii: E37
2017
-
-
1
-
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1
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1
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1
1
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748105
Sutter
Key enzymes of the semiphosph ...
Haloferax volcanii, Haloferax volcanii H26
J. Bacteriol.
198
2251-2262
2016
-
-
1
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2
-
1
1
2
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5
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1
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10
1
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1
1
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1
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2
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1
1
2
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1
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10
1
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1
1
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1
1
-
-
-
726762
Lin
Physiological conditions condu ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
93
1885-1894
2012
-
1
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4
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726975
Cheriyan
Improving upon nature: active ...
Escherichia coli
Biochemistry
51
1658-1668
2012
-
-
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-
4
-
-
14
-
-
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-
-
1
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8
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14
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4
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14
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-
8
-
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14
-
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-
-
14
14
701559
Ryu
Cloning, expression, purificat ...
Zymomonas mobilis, Zymomonas mobilis ZM4
Acta Crystallogr. Sect. F
66
471-473
2010
-
-
1
1
-
-
-
-
-
-
-
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6
-
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1
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2
1
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1
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1
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1
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2
1
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-
704329
Harada
Glucose metabolism in Legionel ...
Legionella pneumophila
J. Bacteriol.
192
2892-2899
2010
-
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3
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1
1
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678625
Walters
Characterization and crystal s ...
Escherichia coli
Bioorg. Med. Chem.
16
710-720
2008
-
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-
2
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3
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2
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2
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3
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690279
Zhang
Cloning, expression, purificat ...
Streptococcus suis
Acta Crystallogr. Sect. F
64
997-999
2008
-
1
1
1
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-
-
-
-
-
3
1
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1
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1
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1
1
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1
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1
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1
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1
1
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-
-
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-
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-
-
691381
Ishikawa
Improvement of L-lysine produc ...
Escherichia coli
Biosci. Biotechnol. Biochem.
72
2535-2542
2008
-
1
1
-
-
-
-
-
-
-
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1
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3
-
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-
-
-
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1
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1
1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695041
Pauluhn
Crystal structure and stereoch ...
Saccharolobus solfataricus, Thermoproteus tenax
Proteins
72
35-43
2008
-
2
2
1
-
-
-
-
-
-
1
6
-
6
-
-
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
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-
-
1
6
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682736
Cheriyan
Mutagenesis of the phosphate-b ...
Escherichia coli
Protein Sci.
16
2368-2377
2007
-
-
1
-
3
-
-
15
-
-
-
-
-
2
-
-
1
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-
1
5
-
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19
-
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1
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3
-
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-
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15
-
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1
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-
1
5
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
664312
Fullerton
Mechanism of the class I KDPG ...
Escherichia coli, Thermotoga maritima
Bioorg. Med. Chem.
14
3002-3010
2006
-
-
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-
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4
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2
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666243
Voss
Application of a KDPG-aldolase ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Metab. Eng.
8
66-78
2006
-
1
-
-
-
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44
-
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682518
Selby
A cryptochrome/photolyase clas ...
Thermotoga maritima
Proc. Natl. Acad. Sci. USA
103
17696-17700
2006
-
-
1
-
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1
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1
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1
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1
2
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1
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1
2
-
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1
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664300
Braga
Slow-binding inhibition of 2-k ...
Escherichia coli
Bioorg. Med. Chem.
12
2965-2972
2004
-
-
-
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-
7
1
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1
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1
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7
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7
7
1
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1
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665573
Theodossis
The structural basis for subst ...
Saccharolobus solfataricus
J. Biol. Chem.
279
43886-43892
2004
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1
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1
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-
2
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649204
Bell
Structure of 2-keto-3-deoxy-6- ...
Pseudomonas putida
Acta Crystallogr. Sect. D
59
1454-1458
2003
-
-
-
1
-
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1
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3
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2
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1
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1
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2
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-
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586786
Griffiths
Cloning, Isolation and Charact ...
Thermotoga maritima
Bioorg. Med. Chem.
10
545-550
2002
-
-
1
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
1
-
10
-
1
-
-
3
-
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-
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1
-
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3
-
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-
1
-
10
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
653666
Allard
Covalent intermediate trapped ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
98
3679-3684
2001
-
-
1
1
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1
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1
1
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1
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1
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1
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1
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1
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-
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-
653924
Wymer
Directed evolution of a new ca ...
Escherichia coli
Structure
9
1-9
2001
-
-
-
1
2
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
1
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3
-
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1
2
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2
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Purification and characterizat ...
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Cloning, characterization and ...
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Metabolism of glucose and gluc ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum CC409, Bradyrhizobium lupini, Bradyrhizobium lupini RL3001, Mesorhizobium loti, Mesorhizobium loti NZP2213, Rhizobium leguminosarum, Rhizobium leguminosarum CC224, Rhizobium leguminosarum SU391, Rhizobium phaseoli, Rhizobium phaseoli CC365, Rhizobium sp., Sinorhizobium meliloti, Sinorhizobium meliloti SU216
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The stereochemistry at carbon ...
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