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Literature summary for 4.1.2.14 extracted from

  • Wood, W.A.
    2-Keto-3-deoxy-6-phosphogluconic and related aldolases (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 7, 281-302.
No PubMed abstract available
Search for more literature for 4.1.2.14

General Stability

General Stability Organism
highly stable when frozen in phosphate buffer Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2-Keto-3-deoxy-6-phosphogalaconate
-
 Pseudomonas putida
2-keto-3-deoxygluconate
-
 Pseudomonas putida
5-Keto-4-deoxyglucarate
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000 78000 disc gel electrophoresis Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pelomonas saccharophila
-
-
-
Pseudomonas putida
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate mechanism Pseudomonas putida
a
2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate Schiff base formation between a lysine epsilon-amino group and carbonyl compounds 6-phospho-2-dehydro-3-deoxy-D-gluconate Pseudomonas putida
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 Pelomonas saccharophila pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-3-deoxy-D-gluconate 6-phosphate highly specific in direction of cleavage Pseudomonas putida pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
additional information catalyzes decarboxylation of oxaloacetate at much slower rate than it cleaves Pseudomonas putida ?
-
 ?
additional information catalyzes exchange of the methyl hydrogen of pyruvate with protons of water Pseudomonas putida ?
-
 ?
additional information catalyzes exchange of the methyl hydrogen of pyruvate with protons of water Pelomonas saccharophila ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 23000-24000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
9
-
 inactivation above Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.7
-
 Pseudomonas putida