Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
D289A | the mutant enzyme exhibits higher resistance against inhibitor (2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine compared to the wild type | Candida albicans |
D289A | compound 3g exhibits similar inhibitory activities to the mutant enzyme (0.0031 mM) as compared to wild-type enzyme | Candida albicans |
R332A | the mutant enzyme exhibits higher resistance against inhibitor (2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine compared to the wild type | Candida albicans |
R332A | mutation results in 60fold (0.101 mM) increase of Ki value for compound 3g compared with wild-type enzyme Ca-FBA-II (0.0017 mM) | Candida albicans |
S62A | the mutant enzyme exhibits higher resistance against inhibitor (2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine compared to the wild type | Candida albicans |
S62A | mutation results in about 15fold increase of the Ki value of S62A (0.0315 mM) as compared to wild-type enzyme | Candida albicans |
T290A | the mutant enzyme exhibits higher resistance against inhibitor (2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine compared to the wild type | Candida albicans |
T290A | mutation results in 130fold (0.230 mM) increase of Ki value for compound 3g as compared with wild-type enzyme Ca-FBA-II (0.0017 mM) | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1E)-1-benzylidene-2-(4-nitrophenyl)hydrazine | 0.05 mM, 43% inhibition; 43% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[(4-chlorophenyl)methylidene]-2-(4-nitrophenyl)hydrazine | 0.05 mM, 91% inhibition; 91% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[(4-fluorophenyl)methylidene]-2-(4-nitrophenyl)hydrazine | 0.05 mM, 94% inhibition; 94% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[(4-methylphenyl)methylidene]-2-(4-nitrophenyl)hydrazine | 0.05 mM, 80% inhibition; 80% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[([1,1'-biphenyl]-4-yl)methylidene]-2-(4-nitrophenyl)hydrazine | 0.05 mM, 92% inhibition; 92% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[1-(4-bromophenyl)ethylidene]-2-(4-nitrophenyl)hydrazine | 0.05 mM, 99% inhibition; 99% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[1-([1,1'-biphenyl]-4-yl)ethylidene]-2-(4-nitrophenyl)hydrazine | 62% inhibition at 0.05 mM | Candida albicans | |
(1E)-1-[2-(4-nitrophenyl)hydrazinylidene]propan-2-one | 0.05 mM, 6% inhibition; 6% inhibition at 0.05 mM | Candida albicans | |
(2E)-1-(4-nitrophenyl)-2-(1-phenylethylidene)hydrazine | 0.05 mM, 62% inhibition | Candida albicans | |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | 0.05 mM, 100% inhibition; complete inhibition at 0.05 mM | Candida albicans | |
(2E)-1-(4-nitrophenyl)-2-[(5-nitrothiophen-2-yl)methylidene]hydrazine | 0.05 mM, 97% inhibition; 97% inhibition at 0.05 mM | Candida albicans | |
(2E)-1-(4-nitrophenyl)-2-[[4-(trifluoromethyl)phenyl]methylidene]hydrazine | 0.05 mM, 60% inhibition; 60% inhibition at 0.05 mM | Candida albicans | |
(2E)-2-[2-(2-hydroxy-4-nitrophenyl)hydrazinylidene]-3-oxobutanamide | 94% inhibition at 0.05 mM | Candida albicans | |
(2E)-2-[2-(4-nitrophenyl)hydrazinylidene]-1-phenylethan-1-one | 0.05 mM, 91% inhibition; 91% inhibition at 0.05 mM | Candida albicans | |
1-(4-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]phenyl)-1H-1,2,4-triazole | 0.05 mM, 83% inhibition; 83% inhibition at 0.05 mM | Candida albicans | |
2-[2-(2,4-dioxopentan-3-ylidene)hydrazinyl]-5-nitrobenzoic acid | 0.05 mM, 10% inhibition; 10% inhibition at 0.05 mM | Candida albicans | |
2-[2-(2,4-dioxopentan-3-ylidene)hydrazinyl]benzonitrile | 0.05 mM, 16% inhibition; 16% inhibition at 0.05 mM | Candida albicans | |
3-(2-phenylhydrazinylidene)pentane-2,4-dione | 0.05 mM, 10% inhibition; 10% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2,4-dinitrophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 1% inhibition; 1% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-chloro-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 68% inhibition; 68% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-fluorophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 3% inhibition; 3% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-hydroxy-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 94% inhibition; 0.05 mM, 96% inhibition; 96% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-hydroxy-5-nitrophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 14% inhibition; 14% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-hydroxyphenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 73% inhibition; 73% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-methyl-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 65% inhibition; 65% inhibition at 0.05 mM | Candida albicans | |
3-[2-(2-methylphenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 9% inhibition; 9% inhibition at 0.05 mM | Candida albicans | |
3-[2-(4-bromophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 27% inhibition; 27% inhibition at 0.05 mM | Candida albicans | |
3-[2-(4-chlorophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 20% inhibition; 20% inhibition at 0.05 mM | Candida albicans | |
3-[2-(4-fluorophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 7% inhibition; 7% inhibition at 0.05 mM | Candida albicans | |
3-[2-(4-hydroxyphenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 5% inhibition; 5% inhibition at 0.05 mM | Candida albicans | |
3-[2-(4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | 0.05 mM, 92% inhibition; 92% inhibition at 0.05 mM | Candida albicans | |
4-nitro-N'-[(E)-(4-nitrophenyl)methylidene]benzohydrazide | 0.05 mM, 59% inhibition; 59% inhibition at 0.05 mM | Candida albicans | |
4-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]benzonitrile | 0.05 mM, 100% inhibition; complete inhibition at 0.05 mM | Candida albicans | |
4-[2-(2,4-dioxopentan-3-ylidene)hydrazinyl]benzene-1-sulfonic acid | 0.05 mM, 14% inhibition; 14% inhibition at 0.05 mM | Candida albicans | |
5-bromo-3-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]-1H-indole | 0.05 mM, 97% inhibition; 97% inhibition at 0.05 mM | Candida albicans | |
N'-[(E)-(4-cyanophenyl)methylidene]-4-nitrobenzohydrazide | 0.05 mM, 21% inhibition; 21% inhibition at 0.05 mM | Candida albicans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Candida albicans | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | Candida albicans ATCC MYA-2876 | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q9URB4 | - |
- |
Candida albicans ATCC MYA-2876 | Q9URB4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Candida albicans | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | - |
Candida albicans ATCC MYA-2876 | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ca-FBA-II | - |
Candida albicans |
class II fructose-1,6-bisphosphate aldolase | - |
Candida albicans |
FBA-II | - |
Candida albicans |
fructose-1,6-bisphosphate aldolase | - |
Candida albicans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | at pH 7.4 and 30°C | Candida albicans | |
0.0017 | - |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | pH and temperature not specified in the publication, wild-type enzyme | Candida albicans | |
0.0031 | - |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | pH and temperature not specified in the publication, mutant enzyme D289A | Candida albicans | |
0.0315 | - |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | pH and temperature not specified in the publication, mutant enzyme S62A | Candida albicans | |
0.101 | - |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | pH and temperature not specified in the publication, mutant enzyme R332A | Candida albicans | |
0.23 | - |
(2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | pH and temperature not specified in the publication, mutant enzyme T290A | Candida albicans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0027 | - |
at pH 7.4 and 30°C | Candida albicans | (2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | |
0.00276 | - |
pH and temperature not specified in the publication, wild-type enzyme | Candida albicans | (2E)-1-(4-nitrophenyl)-2-[(4-nitrophenyl)methylidene]hydrazine | |
0.0041 | - |
pH and temperature not specified in the publication | Candida albicans | (1E)-1-[([1,1'-biphenyl]-4-yl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.0041 | - |
at pH 7.4 and 30°C | Candida albicans | (1E)-1-[([1,1'-biphenyl]-4-yl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.00564 | - |
pH and temperature not specified in the publication | Candida albicans | (1E)-1-[(4-chlorophenyl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.006 | - |
at pH 7.4 and 30°C | Candida albicans | 5-bromo-3-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]-1H-indole | |
0.0064 | - |
at pH 7.4 and 30°C | Candida albicans | (1E)-1-[(4-chlorophenyl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.00643 | - |
pH and temperature not specified in the publication | Candida albicans | 5-bromo-3-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]-1H-indole | |
0.0075 | - |
pH and temperature not specified in the publication | Candida albicans | (1E)-1-[1-(4-bromophenyl)ethylidene]-2-(4-nitrophenyl)hydrazine | |
0.0075 | - |
at pH 7.4 and 30°C | Candida albicans | (1E)-1-[1-(4-bromophenyl)ethylidene]-2-(4-nitrophenyl)hydrazine | |
0.0094 | - |
pH and temperature not specified in the publication | Candida albicans | 4-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]benzonitrile | |
0.0094 | - |
at pH 7.4 and 30°C | Candida albicans | 4-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]benzonitrile | |
0.0126 | - |
pH and temperature not specified in the publication | Candida albicans | (1E)-1-[(4-fluorophenyl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.0126 | - |
at pH 7.4 and 30°C | Candida albicans | (1E)-1-[(4-fluorophenyl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.017 | - |
pH and temperature not specified in the publication | Candida albicans | 3-[2-(2-hydroxy-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | |
0.017 | - |
at pH 7.4 and 30°C | Candida albicans | 3-[2-(2-hydroxy-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | |
0.02 | - |
pH and temperature not specified in the publication | Candida albicans | (1E)-1-[(4-methylphenyl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.02 | - |
pH and temperature not specified in the publication | Candida albicans | (2E)-1-(4-nitrophenyl)-2-[(5-nitrothiophen-2-yl)methylidene]hydrazine | |
0.02 | - |
pH and temperature not specified in the publication | Candida albicans | 3-[2-(2-hydroxy-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | |
0.02 | - |
pH and temperature not specified in the publication | Candida albicans | 3-[2-(4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | |
0.02 | - |
at pH 7.4 and 30°C | Candida albicans | (1E)-1-[(4-methylphenyl)methylidene]-2-(4-nitrophenyl)hydrazine | |
0.02 | - |
at pH 7.4 and 30°C | Candida albicans | (2E)-1-(4-nitrophenyl)-2-[(5-nitrothiophen-2-yl)methylidene]hydrazine | |
0.02 | - |
at pH 7.4 and 30°C | Candida albicans | (2E)-2-[2-(2-hydroxy-4-nitrophenyl)hydrazinylidene]-3-oxobutanamide | |
0.02 | - |
at pH 7.4 and 30°C | Candida albicans | 3-[2-(4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | |
0.025 | - |
pH and temperature not specified in the publication | Candida albicans | 1-(4-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]phenyl)-1H-1,2,4-triazole | |
0.025 | - |
at pH 7.4 and 30°C | Candida albicans | 1-(4-[(E)-[2-(4-nitrophenyl)hydrazinylidene]methyl]phenyl)-1H-1,2,4-triazole | |
0.026 | - |
pH and temperature not specified in the publication | Candida albicans | (2E)-2-[2-(4-nitrophenyl)hydrazinylidene]-1-phenylethan-1-one | |
0.026 | - |
at pH 7.4 and 30°C | Candida albicans | (2E)-2-[2-(4-nitrophenyl)hydrazinylidene]-1-phenylethan-1-one | |
0.038 | - |
pH and temperature not specified in the publication | Candida albicans | 3-[2-(2-hydroxyphenyl)hydrazinylidene]pentane-2,4-dione | |
0.038 | - |
at pH 7.4 and 30°C | Candida albicans | 3-[2-(2-hydroxyphenyl)hydrazinylidene]pentane-2,4-dione | |
0.043 | - |
pH and temperature not specified in the publication | Candida albicans | 3-[2-(2-methyl-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione | |
0.043 | - |
at pH 7.4 and 30°C | Candida albicans | 3-[2-(2-methyl-4-nitrophenyl)hydrazinylidene]pentane-2,4-dione |
General Information | Comment | Organism |
---|---|---|
drug target | the enzyme is an attractive targets for the discovery of drugs to combat invasive fungal infection, because it is absent in animals and higher plants. It is a promising target for the discovery of drugs against azole-resistant fungal pathogens in the future | Candida albicans |