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Literature summary for 4.1.2.13 extracted from
- Isomer activation controls stereospecificity of class I fructose-1,6-bisphosphate aldolases (2017), J. Biol. Chem., 292, 19849-19860 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Star (DE3) cells | Toxoplasma gondii |
| expression in Escherichia coli | Toxoplasma gondii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals are grown at 20°C by hanging-drop vapor diffusion using a 1:1 mixture of protein/precipitant. The enzyme crystallizes with one tetrameric subunit in the asymmetric unit of space group P4(2)2(1)2, adopting the classic (alpha/beta)8-barrel fold | Toxoplasma gondii |
| hanging drop vapor diffusion method, using 0.1 M sodium acetate, pH 5.5, 0.2 M lithium sulfate, 3.5% (w/v) PEG 8000, and 10% (w/v) PEG 1000 | Toxoplasma gondii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tagatose 1,6-bisphosphate | competitive inhibitor | Toxoplasma gondii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00405 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 25°C | Toxoplasma gondii | |
| 0.0045 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 25°C | Toxoplasma gondii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Toxoplasma gondii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate | Toxoplasma gondii | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| D-fructose 1,6-bisphosphate | Toxoplasma gondii | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | Q8I8I2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Toxoplasma gondii |
| HisTrap column chromatography and Superdex gel filtration | Toxoplasma gondii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate | - |
Toxoplasma gondii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| D-fructose 1,6-bisphosphate | - |
Toxoplasma gondii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| Glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
Toxoplasma gondii | D-Fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | x-ray crystallography | Toxoplasma gondii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALD | - |
Toxoplasma gondii |
| class I fructose-1,6-bisphosphate aldolase | - |
Toxoplasma gondii |
| FBP aldolase | - |
Toxoplasma gondii |
| fructose-1,6-bisphosphate aldolase | - |
Toxoplasma gondii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.09 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 25°C | Toxoplasma gondii | |
| 7.09 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 25°C | Toxoplasma gondii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | glycolytic enzyme | Toxoplasma gondii |
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Release 2023.1 (January 2023)
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