Literature summary for 4.1.2.13 extracted from

Capodagli, G.C.; Lee, S.A.; Boehm, K.J.; Brady, K.M.; Pegan, S.D.
Structural and functional characterization of methicillin-resistant Staphylococcus aureus's class IIb fructose 1,6-bisphosphate aldolase (2014), Biochemistry, 53, 7604-7614 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Staphylococcus aureus
expression in Escherichia coli BL21	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion at 20°C. The final crystal condition is comprised of 1.6 M ammonium citrate pH 7.5 and is optimized with 3 M NDSB 195, yielding a 2.1 A data set in a P2(1)2(1)2 space group	Staphylococcus aureus
hanging drop vapor diffusion method, using 1.6 M ammonium citrate (pH 7.5)	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
8-hydroxyquinoline-2-carboxylic acid	mixed type inhibitor	Staphylococcus aureus
citrate	-	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.239	-	D-fructose 1,6-bisphosphate	pH 7.8, temperature not specified in the publication	Staphylococcus aureus
0.239	-	D-fructose 1,6-bisphosphate	at pH 7.8 and 25°C	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains zinc	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 1,6-bisphosphate	Staphylococcus aureus	-	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	Staphylococcus aureus COL	-	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q5HE75	-	-
Staphylococcus aureus COL	Q5HE75	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Staphylococcus aureus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	-	Staphylococcus aureus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	r
D-fructose 1,6-bisphosphate	-	Staphylococcus aureus COL	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	-	Staphylococcus aureus COL	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	r
Glycerone phosphate + D-glyceraldehyde 3-phosphate	-	Staphylococcus aureus	D-Fructose 1,6-bisphosphate	-	?
Glycerone phosphate + D-glyceraldehyde 3-phosphate	-	Staphylococcus aureus COL	D-Fructose 1,6-bisphosphate	-	?

Synonyms

Synonyms	Comment	Organism
class IIb fructose 1,6-bisphosphate aldolase	-	Staphylococcus aureus
FBA	-	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.16	-	D-fructose 1,6-bisphosphate	at pH 7.8 and 25°C	Staphylococcus aureus
1.16	-	D-fructose 1,6-bisphosphate	pH 7.8, temperature not specified in the publication	Staphylococcus aureus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.096	-	8-hydroxyquinoline-2-carboxylic acid	pH 7.8, temperature not specified in the publication	Staphylococcus aureus
0.096	-	8-hydroxyquinoline-2-carboxylic acid	at pH 7.8 and 25°C	Staphylococcus aureus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0569	-	at pH 7.8 and 25°C	Staphylococcus aureus	citrate
0.227	-	at pH 7.8 and 25°C	Staphylococcus aureus	8-hydroxyquinoline-2-carboxylic acid

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Release 2023.1 (January 2023)