Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor phosphoglycolohydroxamate as a mimic of the hydroxyenolate intermediate- and dihydroxyacetone phosphate-bound form of the enzyme, to 1.58 A resolution. Residue E169 facilitates a water-mediated deprotonation-protonation step of the reaction mechanism | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D276A | about 1% of wild-type activity | Mycobacterium tuberculosis |
E168A | about 30% of wild-type activity | Mycobacterium tuberculosis |
E169A | almost complete loss of activity | Mycobacterium tuberculosis |
G167A | about 3% of wild-type activity | Mycobacterium tuberculosis |
G167A/G166A | about 2% of wild-type activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone phosphate | competitive with fructose 1,6-bisphosphate | Mycobacterium tuberculosis | |
Phosphoglycolohydroxamate | use as a mimic of the hydroxyenolate intermediate- and dihydroxyacetone phosphate-bound form of the enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
D-fructose-1,6-bisphosphate | mutant E169A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.021 | - |
D-fructose-1,6-bisphosphate | mutant G167A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.021 | - |
D-fructose-1,6-bisphosphate | mutant G167A/G166A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.03 | - |
D-fructose-1,6-bisphosphate | mutant E168A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.04 | - |
D-fructose-1,6-bisphosphate | wild-type, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.18 | - |
D-fructose-1,6-bisphosphate | mutant D276A, pH 7.8, 22°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQA3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose-1,6-bisphosphate | - |
Mycobacterium tuberculosis | dihydroxyacetone phosphate + glyceraldehyde-3-phosphate | - |
? | |
additional information | product glycerone phosphate is leaving first followed by dihydroxyacetone phosphate in a uni-bi kinetic scheme | Mycobacterium tuberculosis | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
D-fructose-1,6-bisphosphate | mutant E169A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.11 | - |
D-fructose-1,6-bisphosphate | mutant G167A/G166A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.17 | - |
D-fructose-1,6-bisphosphate | mutant G167A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
0.45 | - |
D-fructose-1,6-bisphosphate | mutant D276A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
2.67 | - |
D-fructose-1,6-bisphosphate | mutant E168A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
12 | - |
D-fructose-1,6-bisphosphate | wild-type, pH 7.8, 22°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.238 | - |
dihydroxyacetone phosphate | wild-type, pH 7.8, 22°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
D-fructose-1,6-bisphosphate | mutant E169A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
2.5 | - |
D-fructose-1,6-bisphosphate | mutant D276A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
5.33 | - |
D-fructose-1,6-bisphosphate | mutant G167A/G166A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
8 | - |
D-fructose-1,6-bisphosphate | mutant G167A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
88.33 | - |
D-fructose-1,6-bisphosphate | mutant E168A, pH 7.8, 22°C | Mycobacterium tuberculosis | |
300 | - |
D-fructose-1,6-bisphosphate | wild-type, pH 7.8, 22°C | Mycobacterium tuberculosis |