Literature summary for 4.1.2.13 extracted from

Iturrate, L.; Sanchez-Moreno, I.; Oroz-Guinea, I.; Perez-Gil, J.; Garcia-Junceda, E.
Preparation and characterization of a bifunctional aldolase/kinase enzyme: a more efficient biocatalyst for C-C bond formation (2010), Chemistry, 16, 4018-4030.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Staphylococcus carnosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0146	-	D-fructose 1,6-bisphosphate	native enzyme, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C	Staphylococcus carnosus
0.0188	-	D-fructose 1,6-bisphosphate	recombinant fusion protein DLF, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C	Staphylococcus carnosus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus carnosus	-	-	-
Staphylococcus carnosus CECT 4491	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel/iminodiacetic acid agarose column chromatography and Superdex 75 gel filtration	Staphylococcus carnosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Staphylococcus carnosus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	-	Staphylococcus carnosus CECT 4491	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
DLF	bifunctional enzyme, consists of monomeric fructose-1,6-bisphosphate aldolase from Staphylococcus carnosus and the homodimeric dihydroxyacetone kinase from Citrobacter freundii CECT 4626 with an intervening linker of five amino acid residues	Staphylococcus carnosus
FBPA	-	Staphylococcus carnosus
fructose-1,6-bisphosphate aldolase	-	Staphylococcus carnosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	FBPA shows an almost complete recuperation (92%) of the secondary structure after denaturation at 90°C	Staphylococcus carnosus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
16.76	-	D-fructose 1,6-bisphosphate	native enzyme, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C	Staphylococcus carnosus
20.3	-	D-fructose 1,6-bisphosphate	recombinant fusion protein DLF, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C	Staphylococcus carnosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1080	-	D-fructose 1,6-bisphosphate	recombinant fusion protein DLF, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C	Staphylococcus carnosus
1160	-	D-fructose 1,6-bisphosphate	native enzyme, in Tris-HCl buffer (40 mM, pH 8.0), at 25°C	Staphylococcus carnosus

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Release 2023.1 (January 2023)