Literature summary for 4.1.2.13 extracted from

Rozova, O.N.; Khmelenina, V.N.; Mustakhimov, I.I.; Reshetnikov, A.S.; Trotsenko, Y.A.
Characterization of recombinant fructose-1,6-bisphosphate aldolase from Methylococcus capsulatus Bath (2010), Biochemistry, 75, 892-898.

Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Methylococcus capsulatus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	complete inhibition at 1 mM	Methylococcus capsulatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0052	-	D-fructose 1,6-bisphosphate	in the presence of 0.02 mM Co2+, at substrate concentrations of 0.008-0.5 mM, at pH 7.5, 30°C	Methylococcus capsulatus
0.018	-	D-fructose 1,6-bisphosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
0.095	-	dihydroxyacetone phosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
0.19	-	sedoheptulose 1,7-bisphosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
1.1	-	D-fructose 1,6-bisphosphate	in the presence of 0.02 mM Co2+, at substrate concentrations of 0.5-1.5 mM, at pH 7.5, 30°C	Methylococcus capsulatus
1.17	-	D-glyceraldehyde 3-phosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	1 mM lowers enzyme activity by 90%	Methylococcus capsulatus
Co2+	the enzyme activity increases 2.5times in the presence of Co2+	Methylococcus capsulatus
Cu2+	1 mM lowers enzyme activity by 80%	Methylococcus capsulatus
additional information	Mg2+ or Ca2+ have no effect on enzme activity	Methylococcus capsulatus
Zn2+	1 mM lowers enzyme activity by 60%	Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38500	-	6 * 38500, calculated from amino acid sequence	Methylococcus capsulatus
40000	-	6 * 40000, SDS-PAGE	Methylococcus capsulatus
240000	-	gel filtration	Methylococcus capsulatus

Organism

Organism	UniProt	Comment	Textmining
Methylococcus capsulatus	Q602L6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA column chromatography	Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Methylococcus capsulatus	dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate	-	r
dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate	-	Methylococcus capsulatus	D-fructose 1,6-bisphosphate	-	r
sedoheptulose 1,7-bisphosphate	-	Methylococcus capsulatus	glycerone phosphate + D-erythrose 4-phosphate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 40000, SDS-PAGE	Methylococcus capsulatus
homohexamer	6 * 38500, calculated from amino acid sequence	Methylococcus capsulatus

Synonyms

Synonyms	Comment	Organism
FBA	the Methylococcus capsulatus enzyme belongs to the type B of class II fructose-1,6-bisphosphate aldolases	Methylococcus capsulatus
fructose-1,6-bisphosphate aldolase	-	Methylococcus capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	80	the enzyme activity is retained after incubation for 2 h at 30, 40, or 50°C, respectively. The half-life of the enzyme is 1 h at 60°C, and the enzyme is fully inactivated after heating at 80°C for 10 min	Methylococcus capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.002	-	D-glyceraldehyde 3-phosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
0.002	-	dihydroxyacetone phosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
0.003	-	sedoheptulose 1,7-bisphosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
0.005	-	D-fructose 1,6-bisphosphate	in the absence of Co2+, at pH 7.5, 30°C	Methylococcus capsulatus
0.018	-	D-fructose 1,6-bisphosphate	in the presence of 0.02 mM Co2+, at substrate concentrations of 0.008-0.5 mM, at pH 7.5, 30°C	Methylococcus capsulatus
0.055	-	D-fructose 1,6-bisphosphate	in the presence of 0.02 mM Co2+, at substrate concentrations of 0.5-1.5 mM, at pH 7.5, 30°C	Methylococcus capsulatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	9.5	-	Methylococcus capsulatus

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Release 2023.1 (January 2023)