Literature summary for 4.1.2.13 extracted from

St-Jean, M.; Blonski, C.; Sygusch, J.
Charge stabilization and entropy reduction of central lysine residues in fructose-bisphosphate aldolase (2009), Biochemistry, 48, 4528-4537.

Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 SI cells	Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
D33N and D33S mutant enzymes	Oryctolagus cuniculus

Protein Variants

Protein Variants	Comment	Organism
D33N	the mutation drastically reduces the rate of turnover but does not impact substrate binding	Oryctolagus cuniculus
D33S	the mutation drastically reduces the rate of turnover but does not impact substrate binding	Oryctolagus cuniculus
E187A	the mutation drastically reduces the rate of turnover	Oryctolagus cuniculus
E187Q	the mutation drastically reduces the rate of turnover	Oryctolagus cuniculus
K107M	the mutation drastically reduces the rate of turnover	Oryctolagus cuniculus
K146A	the mutation drastically reduces the rate of turnover	Oryctolagus cuniculus
K146M	the mutation drastically reduces the rate of turnover	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0042	-	D-fructose 1,6-bisphosphate	mutant enzyme D33N	Oryctolagus cuniculus
0.0051	-	D-fructose 1,6-bisphosphate	native wild type enzyme	Oryctolagus cuniculus
0.0061	-	D-fructose 1,6-bisphosphate	mutant enzyme E187A	Oryctolagus cuniculus
0.0084	-	D-fructose 1,6-bisphosphate	mutant enzyme D33S	Oryctolagus cuniculus
0.0134	-	D-fructose 1,6-bisphosphate	mutant enzyme E187Q	Oryctolagus cuniculus
0.0204	-	D-fructose 1,6-bisphosphate	mutant enzyme K146M	Oryctolagus cuniculus
0.0412	-	D-fructose 1,6-bisphosphate	mutant enzyme K107M	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	P00883	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Oryctolagus cuniculus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
FBP aldolase	-	Oryctolagus cuniculus
fructose-1,6-bisphosphate muscle aldolase	-	Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0002	-	D-fructose 1,6-bisphosphate	turnover number less than 0.0002 sec-1, mutant enzyme K146A	Oryctolagus cuniculus
0.0019	-	D-fructose 1,6-bisphosphate	mutant enzyme K146M	Oryctolagus cuniculus
0.002	-	D-fructose 1,6-bisphosphate	mutant enzyme D33S	Oryctolagus cuniculus
0.003	-	D-fructose 1,6-bisphosphate	mutant enzyme D33N	Oryctolagus cuniculus
0.005	-	D-fructose 1,6-bisphosphate	mutant enzyme E187Q	Oryctolagus cuniculus
0.046	-	D-fructose 1,6-bisphosphate	mutant enzyme E187A	Oryctolagus cuniculus
0.65	-	D-fructose 1,6-bisphosphate	mutant enzyme K107M	Oryctolagus cuniculus
13.22	-	D-fructose 1,6-bisphosphate	native wild type enzyme	Oryctolagus cuniculus

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Release 2023.1 (January 2023)