Cloned (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes G346S and E206K, expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E206K | mutant has a KM-value 2fold higher than that of both the Gly346S mutant and the wild-type enzyme, and a turnover-number value 40% less than the wild-type | Homo sapiens |
G346S | mutant enzyme has the same KM-value as the wild-type enzyme, but a 4fold lower turnover-number | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0519 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme | Homo sapiens | |
0.0566 | - |
D-fructose 1,6-bisphosphate | mutant enzyme G346S | Homo sapiens | |
0.105 | - |
D-fructose 1,6-bisphosphate | mutant enzyme E206K | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04075 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes G346S and E206K | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.1 | - |
mutant enzyme G346S | Homo sapiens |
15.7 | - |
mutant enzyme E206K | Homo sapiens |
22.3 | - |
wild-type enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 50% v/v glycerol/20 mM TrisHCl, stable for at least 1 month | Homo sapiens |
4°C, 20 mM Tris/HCl, pH 7.4, stable for at least 2 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Homo sapiens | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
melting temperature of mutant E206K | Homo sapiens |
54 | - |
melting temperature of wild-type enzyme is 54.4°C | Homo sapiens |
55 | - |
melting temperature of mutant enzyme G346S is 54.8°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
D-fructose 1,6-bisphosphate | mutant enzyme G346S | Homo sapiens | |
10.7 | - |
D-fructose 1,6-bisphosphate | mutant enzyme E206K | Homo sapiens | |
16.7 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme | Homo sapiens |