Literature summary for 4.1.2.13 extracted from

Plater, A.R.; Zgiby, S.M.; Thomson, G.J.; Qamar, S.; Wharton, C.W.; Berry, A.
Conserved residues in the mechanism of the E. coli Class II FBP-aldolase (1999), J. Mol. Biol., 285, 843-855.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D109A	very low catalytic activity	Escherichia coli
D144A	increased Km	Escherichia coli
D288A	increased Km	Escherichia coli
D290A	increased Km	Escherichia coli
D329A	increased Km	Escherichia coli
N286A	increased Km, decreased kcat	Escherichia coli
N286D	increased Km, severely decreased kcat	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
dihydroxyacetone phosphate	-	Escherichia coli
glyceraldehyde 3-phosphate	non-competitive inhibitor	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	D-fructose 1,6-bisphosphate	30°C, D109A mutant	Escherichia coli
0.19	-	D-fructose 1,6-bisphosphate	30°C, wild-type enzyme	Escherichia coli
0.37	-	D-fructose 1,6-bisphosphate	30°C, N286D mutant	Escherichia coli
0.77	-	D-fructose 1,6-bisphosphate	30°C, D290A mutant	Escherichia coli
0.92	-	D-fructose 1,6-bisphosphate	30°C, D329A mutant	Escherichia coli
0.94	-	D-fructose 1,6-bisphosphate	30°C, D144A mutant	Escherichia coli
1	-	D-fructose 1,6-bisphosphate	30°C, D288A mutant	Escherichia coli
1.07	-	D-fructose 1,6-bisphosphate	30°C, N286A mutant	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 1,6-bisphosphate	Escherichia coli	-	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AB71	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate	mechanism of class II aldolase	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Escherichia coli	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.001	-	D-fructose 1,6-bisphosphate	30°C, N286D mutant	Escherichia coli
0.0028	-	D-fructose 1,6-bisphosphate	30°C, D109A mutant	Escherichia coli
0.19	-	D-fructose 1,6-bisphosphate	30°C, N286A mutant	Escherichia coli
2	-	D-fructose 1,6-bisphosphate	30°C, D144A mutant	Escherichia coli
2.2	-	D-fructose 1,6-bisphosphate	30°C, D288A mutant	Escherichia coli
7	-	D-fructose 1,6-bisphosphate	30°C, D290A mutant	Escherichia coli
8.2	-	D-fructose 1,6-bisphosphate	30°C, wild-type enzyme	Escherichia coli
12.3	-	D-fructose 1,6-bisphosphate	30°C, D329A mutant	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.13	-	dihydroxyacetone phosphate	30°C, wild-type enzyme	Escherichia coli
1.7	-	dihydroxyacetone phosphate	30°C, N268A mutant	Escherichia coli

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Release 2023.1 (January 2023)