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Literature summary for 4.1.2.13 extracted from

  • Siebers, B.; Brinkmann, H.; Dorr, C.; Tjaden, B.; Lilie, H.; van der Oost, J.; Verhees, C.H.
    Archaeal fructose-1,6-bisphosphate aldolases constitute a new family of archaeal type class I aldolase (2001), J. Biol. Chem., 276, 28710-28718.
    View publication on PubMed
Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermoproteus tenax
expressed in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 fructose 1,6-bisphosphate 50°C, pH 7 Thermoproteus tenax
0.009
-
 fructose 1,6-bisphosphate 50°C, pH 7 Pyrococcus furiosus
0.71
-
 fructose 1-phosphate 50°C, pH 7 Thermoproteus tenax
4.48
-
 fructose 1-phosphate 50°C, pH 7 Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28700
-
 8 * 28700, SDS-PAGE Thermoproteus tenax
31100
-
 8 * 31100, SDS-PAGE Pyrococcus furiosus
241000
-
 gel filtration Thermoproteus tenax
272000
-
 gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P58314
-
-
Thermoproteus tenax P58315
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermoproteus tenax
recombinant protein Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate preferred substrate, reaction intermediate is Schiff base Thermoproteus tenax glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
D-fructose 1,6-bisphosphate preferred substrate, reaction intermediate is Schiff base Pyrococcus furiosus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
D-Fructose 1-phosphate
-
 Thermoproteus tenax Glycerone phosphate + D-glyceraldehyde
-
 ?
D-Fructose 1-phosphate
-
 Pyrococcus furiosus Glycerone phosphate + D-glyceraldehyde
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 28700, SDS-PAGE Thermoproteus tenax
octamer 8 * 31100, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Thermoproteus tenax
50
-
 assay at Pyrococcus furiosus