Application | Comment | Organism |
---|---|---|
medicine | aldolase B deficiency causes hereditary fructose intolerance | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A149P | no catalytic activity | Homo sapiens |
L256P | melting temperature is 40°C versus 47°C for the wild-type enzyme | Homo sapiens |
N334K | increased Km value | Homo sapiens |
Q354E | kinetis resemble that of the wild-type enzyme | Homo sapiens |
W147R | melting temperature is 40°C versus 47°C for the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
D-Fructose 1-phosphate | W147R mutant, 22°C | Homo sapiens | |
0.00173 | - |
D-Fructose 1-phosphate | L256P mutant, 22°C | Homo sapiens | |
0.0022 | - |
D-Fructose 1-phosphate | wild-type enzyme, 22°C | Homo sapiens | |
0.0023 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 22°C | Homo sapiens | |
0.0055 | - |
D-fructose 1,6-bisphosphate | W147R mutant, 22°C | Homo sapiens | |
0.0073 | - |
D-fructose 1,6-bisphosphate | L256P mutant, 22°C | Homo sapiens | |
0.0091 | - |
D-fructose 1,6-bisphosphate | Q354E mutant, 22°C | Homo sapiens | |
0.022 | - |
D-Fructose 1-phosphate | N334K mutant, 22°C | Homo sapiens | |
0.032 | - |
D-fructose 1,6-bisphosphate | N334K mutant, 22°C | Homo sapiens | |
0.05 | - |
D-Fructose 1-phosphate | Q354E mutant, 22°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
tetrameric form, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Homo sapiens | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05062 | - |
- |
Purification (Comment) | Organism |
---|---|
95% homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
N334K mutant, D-fructose 1-phosphate as substrate | Homo sapiens |
0.28 | - |
N334K mutant, D-fructose 1,6-bisphosphate as substrate | Homo sapiens |
0.38 | - |
W147R mutant, D-fructose 1-phosphate as substrate | Homo sapiens |
0.58 | - |
L256P mutant, D-fructose 1-phosphate as substrate | Homo sapiens |
0.64 | - |
W147R mutant, D-fructose 1,6-bisphosphate as substrate | Homo sapiens |
0.81 | - |
L256P mutant, D-fructose 1,6-bisphosphate as substrate | Homo sapiens |
0.86 | - |
Q354E mutant, D-fructose 1,6-bisphosphate as substrate | Homo sapiens |
0.88 | - |
Q354E mutant, D-fructose 1-phosphate as substrate | Homo sapiens |
0.94 | - |
wild-type enzyme, D-fructose 1-phosphate as substrate | Homo sapiens |
0.97 | - |
wild-type enzyme, D-fructose 1,6-bisphosphate as substrate | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, wild-type and Q354E mutant: stable in 50% glycerol for 1 month, W147R and L256P mutant enzyme: aggregate after 15 days suggesting structural instability | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Homo sapiens | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-Fructose 1-phosphate | - |
Homo sapiens | Glycerone phosphate + D-glyceraldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | A149P mutant shows tetrameric and monomeric forms and other species with molecular masses intermediate between those corresponding to the tetramer and monomer | Homo sapiens |
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aldolase B | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
W147R melting temperature of W147R mutant and L256P mutant is 40°C versus 47°C for the wild-type enzyme | Homo sapiens |
47 | - |
melting temperature of the wild-type enzyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
D-Fructose 1-phosphate | Q354E mutant, 22°C | Homo sapiens | |
0.085 | - |
D-Fructose 1-phosphate | N334K mutant, 22°C | Homo sapiens | |
0.19 | - |
D-fructose 1,6-bisphosphate | N334K mutant, 22°C | Homo sapiens | |
0.25 | - |
D-Fructose 1-phosphate | W147R mutant, 22°C | Homo sapiens | |
0.38 | - |
D-Fructose 1-phosphate | L256P mutant, 22°C | Homo sapiens | |
0.43 | - |
D-fructose 1,6-bisphosphate | W147R mutant, 22°C | Homo sapiens | |
0.54 | - |
D-fructose 1,6-bisphosphate | L256P mutant, 22°C | Homo sapiens | |
0.57 | - |
D-fructose 1,6-bisphosphate | Q354E mutant, 22°C | Homo sapiens | |
0.61 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 22°C | Homo sapiens | |
0.67 | - |
D-Fructose 1-phosphate | wild-type enzyme, 22°C | Homo sapiens | |
6.08 | - |
D-fructose 1,6-bisphosphate | L256P mutant, 22°C | Homo sapiens | |
6.08 | - |
D-fructose 1,6-bisphosphate | Q354E mutant, 22°C | Homo sapiens | |
6.08 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 22°C | Homo sapiens | |
6.08 | - |
D-Fructose 1-phosphate | wild-type enzyme, 22°C | Homo sapiens |