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Literature summary for 4.1.2.13 extracted from

  • MacDonald, J.A.; Storey, K.B.
    Purification and characterization of fructose bisphosphate aldolase from the ground squirrel, Spermophilus lateralis: enzyme role in mammalian hibernation (2002), Arch. Biochem. Biophys., 408, 279-285.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 50% inhibition at 2.1 mM at 37°C, 50% inhibition at 1.9 mM at 5°C Callospermophilus lateralis
(NH4)2SO4 50% inhibition at 6.2 mM at 37°C, 50% inhibition at 5.27 mM at 5°C Oryctolagus cuniculus
ADP 50% inhibition at 1.6 mM at 37°C, 50% inhibition at 1.7 mM at 5°C Callospermophilus lateralis
ADP 50% inhibition at 2.7 mM at 37°C, 50% inhibition at 5.4 mM at 5°C Oryctolagus cuniculus
AMP 50% inhibition at 2.3 mM at 37°C, 50% inhibition at 2.6 mM at 5°C Callospermophilus lateralis
AMP 50% inhibition at 4.5 mM at 37°C, 50% inhibition at 6.0 mM at 5°C Oryctolagus cuniculus
ATP 50% inhibition at 1.4 mM at 37°C, 50% inhibition at 1.1 mM at 5°C Callospermophilus lateralis
ATP 50% inhibition at 2.1 mM at 37°C, 50% inhibition at 2.1 mM at 5°C Oryctolagus cuniculus
citrate 50% inhibition at 2.4 mM at 37°C, 50% inhibition at 1.9 mM at 5°C Callospermophilus lateralis
citrate 50% inhibition at 2.7 mM at 37°C, 50% inhibition at 3.4 mM at 5°C Oryctolagus cuniculus
IMP 50% inhibition at 1.3 mM at 37°C, 50% inhibition at 1.3 mM at 5°C Callospermophilus lateralis
IMP 50% inhibition at 4.2 mM at 37°C, 50% inhibition at 8.5 mM at 5°C Oryctolagus cuniculus
KCl 50% inhibition at 38.2 mM at 37°C, 50% inhibition at 36.1 mM at 5°C Callospermophilus lateralis
KCl 50% inhibition at 111 mM at 37°C, 50% inhibition at 63.3 mM at 5°C Oryctolagus cuniculus
Magnesium citrate 50% inhibition at 1.9 mM at 37°C, 50% inhibition at 2.5 mM at 5°C Callospermophilus lateralis
Magnesium citrate 50% inhibition at 9 mM at 37°C, 50% inhibition at 2.5 mM at 5°C Oryctolagus cuniculus
NaCl 50% inhibition at 14.7 mM at 37°C, 50% inhibition at 31 mM at 5°C Callospermophilus lateralis
NaCl 50% inhibition at 110 mM at 37°C, 50% inhibition at 52 mM at 5°C Oryctolagus cuniculus
NH4Cl 50% inhibition at 51.5 mM at 37°C, 50% inhibition at 34.1 mM at 5°C Callospermophilus lateralis
NH4Cl 50% inhibition at 166 mM at 37°C, 50% inhibition at 40.6 mM at 5°C Oryctolagus cuniculus
phosphate 50% inhibition at 1.7 mM at 37°C, 50% inhibition at 0.63 mM at 5°C Callospermophilus lateralis
phosphate 50% inhibition at 6.1 mM at 37°C, 50% inhibition at 1.7 mM at 5°C Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
D-fructose 1,6-bisphosphate pH 7.3, 37°C Callospermophilus lateralis
0.0013
-
D-fructose 1,6-bisphosphate pH 7.3, 37°C Oryctolagus cuniculus
0.00145
-
D-fructose 1,6-bisphosphate pH 7.3, 5°C Callospermophilus lateralis
0.00411
-
D-fructose 1,6-bisphosphate pH 7.3, 5°C Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Callospermophilus lateralis
159000
-
gel filtration Callospermophilus lateralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate Oryctolagus cuniculus
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r
D-fructose 1,6-bisphosphate Callospermophilus lateralis
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Callospermophilus lateralis
-
golden-mantled ground squirrel
-
Oryctolagus cuniculus
-
rabbit
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Callospermophilus lateralis

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Callospermophilus lateralis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Oryctolagus cuniculus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r
D-fructose 1,6-bisphosphate
-
Callospermophilus lateralis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Callospermophilus lateralis

Synonyms

Synonyms Comment Organism
F1,6P2 aldolase
-
Callospermophilus lateralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 7.5
-
Callospermophilus lateralis
7
-
-
Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
steep drop in activity above Callospermophilus lateralis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00023
-
phosphate pH 7.3, 5°C Callospermophilus lateralis
0.00118
-
phosphate pH 7.3, 37°C Callospermophilus lateralis

pI Value

Organism Comment pI Value Maximum pI Value
Callospermophilus lateralis isoelectric focusing, pH gradient pH 3.5 - pH 10
-
5.5