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Literature summary for 4.1.2.13 extracted from
- Functional characterization of an extreme thermophilic class II fructose-1,6-bisphosphate aldolase (1996), Eur. J. Biochem., 241, 243-248.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 10% w/v, increases activity by 24% | Thermus aquaticus |  |
| Nonidet-P40 | 0.5% w/v, enhances activity by 34% | Thermus aquaticus | |
| Triton X-100 | 0.5% w/v, enhances activity by 31% | Thermus aquaticus | |
| Tween 20 | 0.5% w/v, enhances activity by 88% | Thermus aquaticus | |
| Tween 80 | 0.5% w/v, enhances activity by 39% | Thermus aquaticus | |
General Stability
| General Stability | Organism |
|---|---|
| 10% w/v ethanol results in 2fold reduction in thermal stability | Thermus aquaticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butanol | - |
Thermus aquaticus | |
| EDTA | - |
Thermus aquaticus | |
| Propanol | - |
Thermus aquaticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Thermus aquaticus | |
| Fe2+ | activates | Thermus aquaticus | |
| Zn2+ | activates | Thermus aquaticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
4 * 34000, SDS-PAGE | Thermus aquaticus |
| 165000 | - |
- |
Thermus aquaticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus aquaticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus aquaticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
- |
Thermus aquaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate | - |
Thermus aquaticus | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 34000, SDS-PAGE | Thermus aquaticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
10% w/v ethanol results in 2fold reduction in thermal stability | Thermus aquaticus |
| 80 | - |
2 h, stable | Thermus aquaticus |
| 90 | - |
2 h, stable | Thermus aquaticus |
| 90 | 93 | half-life: 2 h | Thermus aquaticus |
| 95 | - |
half-life: 46 min | Thermus aquaticus |
| 97 | - |
1 h, 80% loss of activity. Half-life: 16 min | Thermus aquaticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermus aquaticus |
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