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Literature summary for 4.1.2.13 extracted from

  • Kusakabe, T.; Motoki, K.; Hori, K.
    Human aldolase C: characterization of the recombinant enzyme expressed in Escherichia coli (1994), J. Biochem., 115, 1172-1177.
    View publication on PubMed
Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment) Organism
aldolase C, expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y363S Y363S has reduced catalytic activity towards D-fructose 1,6-bisphosphate and fructose 1-phosphate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 D-fructose 1,6-bisphosphate mutant aldolase C Y363S Homo sapiens
0.0089
-
 D-fructose 1,6-bisphosphate aldolase C Rattus norvegicus
0.012
-
 D-fructose 1,6-bisphosphate aldolase B Homo sapiens
0.013
-
 D-fructose 1,6-bisphosphate aldolase C Homo sapiens
0.052
-
 D-fructose 1,6-bisphosphate aldolase A Homo sapiens
1.7
-
 fructose 1-phosphate mutant aldolase C Y363S Homo sapiens
3.7
-
 fructose 1-phosphate aldolase B Homo sapiens
18
-
 fructose 1-phosphate aldolase C Homo sapiens
25
-
 fructose 1-phosphate aldolase C Rattus norvegicus
27
-
 fructose 1-phosphate aldolase A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
 Homo sapiens glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-fructose 1,6-bisphosphate
-
 Rattus norvegicus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-Fructose 1-phosphate
-
 Rattus norvegicus Glycerone phosphate + D-glyceraldehyde
-
 r
D-Fructose 1-phosphate recombinant enzyme: 7.4% of the activity with fructose 1,6-diphosphate Homo sapiens Glycerone phosphate + D-glyceraldehyde
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, about 80% loss of aldolase C activity with fructose 1-phosphate as substrate Homo sapiens
50
-
 30 min, about 70% loss of aldolase B activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens
50
-
 30 min, about 5% loss of aldolase A activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens
50
-
 30 min, about 40% loss of aldolase C activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens
60
-
 30 min, about 90% loss of aldolase A activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.75
-
 fructose 1-phosphate mutant aldolase C Y363S Homo sapiens
1.28
-
 fructose 1-phosphate aldolase A Homo sapiens
1.48
-
 fructose 1-phosphate aldolase C Homo sapiens
2.67
-
 fructose 1-phosphate aldolase C Rattus norvegicus
2.83
-
 D-fructose 1,6-bisphosphate mutant aldolase C Y363S Homo sapiens
10.8
-
 fructose 1-phosphate aldolase B Homo sapiens
12.6
-
 D-fructose 1,6-bisphosphate aldolase C Homo sapiens
20.1
-
 D-fructose 1,6-bisphosphate aldolase B Homo sapiens
33
-
 D-fructose 1,6-bisphosphate aldolase C Rattus norvegicus
59.7
-
 D-fructose 1,6-bisphosphate aldolase A Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 9
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 12 pH 4: about 80% of maximal activity, pH 12: about 75% of maximal activity Homo sapiens