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Literature summary for 4.1.2.11 extracted from

  • Guterl, J.K.
    Andexer, J.N.; Sehl, T.; von Langermann, J.; Frindi-Wosch, I.; Rosenkranz, T.; Fitter, J.; Gruber, K.; Kragl, U.; Eggert, T.; Pohl, M.: Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold (2009), J. Biotechnol., 141, 166-173.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1
-
(S)-4-hydroxymandelonitrile pH 5.0, 25°C Manihot esculenta
5.9
-
4-hydroxybenzaldehyde pH 5.0, 25°C Manihot esculenta
179
-
cyanide pH 5.0, 25°C Manihot esculenta

Organism

Organism UniProt Comment Textmining
Manihot esculenta
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-4-Hydroxymandelonitrile
-
Manihot esculenta Cyanide + 4-hydroxybenzaldehyde
-
r
cyanide + 4-hydroxybenzaldehyde
-
Manihot esculenta (S)-4-hydroxymandelonitrile
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Manihot esculenta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
20 stable Manihot esculenta
37
-
half-life 64 h Manihot esculenta
50
-
half-life 2.7 h Manihot esculenta
60
-
half-life 0.5 h Manihot esculenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
Manihot esculenta

pH Range

pH Minimum pH Maximum Comment Organism
4
-
40% of maxinmum activity Manihot esculenta