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Literature summary for 4.1.2.11 extracted from
- The metabolism of aromatic compounds in higher plants. VII. On the requirement of hydroxynitrile lyase for plants (1966), J. Biol. Chem., 241, 4457-4462.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by iodoacetamide, p-chloromercuribenzoate, 2,2'-dipyridyl, 1,10-phenanthroline, phenylthiourea, NaCN, FeSO4, Na2S | Sorghum bicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
D,L-hydroxymandelonitrile | - |
Sorghum bicolor |  |
| 0.73 | - |
D,L-vanillin cyanohydrin | - |
Sorghum bicolor | |
| 0.79 | - |
DL-mandelonitrile | - |
Sorghum bicolor | |
| 1.2 | - |
D,L-isovanillin cyanohydrin | - |
Sorghum bicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for metal ions | Sorghum bicolor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
sedimentation equilibrium centrifugation | Sorghum bicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorghum bicolor | - |
i.e. Sorghum vulgare | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sorghum bicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Sorghum bicolor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 188 | - |
4-hydroxymandelonitrile | Sorghum bicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-4-Hydroxymandelonitrile | - |
Sorghum bicolor | Cyanide + 4-hydroxybenzaldehyde | - |
? | |
| 4-Hydroxymandelonitrile | - |
Sorghum bicolor | ? | - |
? | |
| DL-mandelonitrile | - |
Sorghum bicolor | cyanide + benzaldehyde | - |
? | |
| isovanillin cyanohydrin | - |
Sorghum bicolor | cyanide + isovanillin | - |
? | |
| vanillin cyanohydrin | - |
Sorghum bicolor | cyanide + vanillin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sorghum hydroxynitrile lyase | - |
Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Sorghum bicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6.5 | - |
Sorghum bicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Sorghum bicolor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no flavin group | Sorghum bicolor |
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Release 2023.1 (January 2023)
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