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Literature summary for 4.1.2.11 extracted from
- Metabolism of aromatic compounds in higher plants. II. Purification and properties of the oxynitrilase of Sorghum vulgare (1961), J. Biol. Chem., 236, 207-210.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Sorghum bicolor |  |
| Fe2(SO4)3 | - |
Sorghum bicolor | |
| HgCl2 | - |
Sorghum bicolor | |
| additional information | not inhibited by iodoacetamide, p-chloromercuribenzoate, 2,2'-dipyridyl, 1,10-phenanthroline, phenylthiourea, NaCN, FeSO4, Na2S | Sorghum bicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
4-hydroxymandelonitrile | - |
Sorghum bicolor | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-Hydroxymandelonitrile | Sorghum bicolor | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorghum bicolor | - |
i.e. Sorghum vulgare | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sorghum bicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epicotyl | - |
Sorghum bicolor | - |
| seedling | - |
Sorghum bicolor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Sorghum bicolor |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, no loss of activity after 3 months | Sorghum bicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-4-Hydroxymandelonitrile | inactive against 2-hydroxymanelonitrile or 3-hydroxymandelonitrile | Sorghum bicolor | Cyanide + 4-hydroxybenzaldehyde | - |
? | |
| (S)-mandelonitrile | inactive | Sorghum bicolor | cyanide + benzaldehyde | - |
? | |
| 4-Hydroxymandelonitrile | - |
Sorghum bicolor | ? | - |
? | |
| vanillin cyanohydrin | 20% the rate of 4-hydroxybenzaldehyde | Sorghum bicolor | cyanide + vanillin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Sorghum bicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
thermal inactivation above | Sorghum bicolor |
| 50 | - |
60% loss of activity after 5 min, protein impurities in crude extract protect | Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Sorghum bicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | 6.3 | - |
Sorghum bicolor |
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Release 2023.1 (January 2023)
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