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Literature summary for 4.1.2.10 extracted from
- Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis (2015), Proc. Natl. Acad. Sci. USA, 112, 10605-10610 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the high specific activity toward benzaldehyde along with wide temperature, pH stabilities and high enantioselectivity in the synthesis of various cyanohydrins make this enzyme suitable as an industrial biocatalyst | Chamberlinius hualienensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
2,4-dimethylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis |  |
| 3 | - |
benzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 5.4 | - |
4-methylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 7.5 | - |
4-methoxybenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 7.7 | - |
3-Methoxybenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 10 | - |
4-bromobenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 10.3 | - |
4-biphenylcarboxaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 10.5 | - |
3-methylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 18 | - |
2-thiophenecarboxaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47300 | - |
gel filtration | Chamberlinius hualienensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chamberlinius hualienensis | A0A0H5BR52 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Chamberlinius hualienensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chamberlinius hualienensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7420 | - |
pH 4.2, 22°C | Chamberlinius hualienensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanide + 2,4-dimethylbenzaldehyde | - |
Chamberlinius hualienensis | (R)-2-hydroxy-2-(2,4-dimethylphenyl)acetonitrile | - |
? | |
| cyanide + 2-thiophenecarboxaldehyde | - |
Chamberlinius hualienensis | ? | - |
? | |
| cyanide + 3-methoxybenzaldehyde | - |
Chamberlinius hualienensis | (2R)-(3-methoxyphenyl)(hydroxy)ethanenitrile | - |
? | |
| cyanide + 3-methylbenzaldehyde | - |
Chamberlinius hualienensis | (2R)-(3-methylphenyl)(hydroxy)ethanenitrile | - |
? | |
| cyanide + 4-biphenylcarboxaldehyde | - |
Chamberlinius hualienensis | ? | - |
? | |
| cyanide + 4-bromobenzaldehyde | - |
Chamberlinius hualienensis | (2R)-(4-bromophenyl)(hydroxy)ethanenitrile | - |
? | |
| cyanide + 4-methoxybenzaldehyde | - |
Chamberlinius hualienensis | (2R)-(4-methoxyphenyl)(hydroxy)ethanenitrile | - |
? | |
| cyanide + 4-methylbenzaldehyde | - |
Chamberlinius hualienensis | (2R)-(4-methylphenyl)(hydroxy)ethanenitrile | - |
? | |
| cyanide + benzaldehyde | the enzyme catalyzes the synthesis of (R)-mandelonitrile from benzaldehyde with a 99% enantiomeric excess | Chamberlinius hualienensis | (R)-mandelonitrile | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 25000, SDS-PAGE | Chamberlinius hualienensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Chamberlinius hualienensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 35% of maximal activity, 50°C: about 60% of maximal activity | Chamberlinius hualienensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 40 | 1 h, no loss of activity | Chamberlinius hualienensis |
| 65 | - |
1 h, 10% loss of activity | Chamberlinius hualienensis |
| 70 | - |
1 h, 40% loss of activity | Chamberlinius hualienensis |
| 75 | - |
1 h, complete loss of activity | Chamberlinius hualienensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 49 | - |
4-biphenylcarboxaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 300 | - |
2,4-dimethylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 1490 | - |
2-thiophenecarboxaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 1680 | - |
3-Methoxybenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 3390 | - |
benzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 3480 | - |
4-methoxybenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 5520 | - |
4-methylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 6890 | - |
3-methylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 8610 | - |
4-bromobenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Chamberlinius hualienensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 5.3 | pH 4.3: about 45% of maximal activity, pH 5.3: about 50% of maximal activity | Chamberlinius hualienensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
1 h, about % loss of activity | Chamberlinius hualienensis |
| 5.5 | - |
1 h, about 20% loss of activity | Chamberlinius hualienensis |
| 8 | 10 | 1 h, no loss of activity | Chamberlinius hualienensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Chamberlinius hualienensis | calculated from sequence | - |
4.97 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
4-biphenylcarboxaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 83 | - |
2-thiophenecarboxaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 220 | - |
3-Methoxybenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 230 | - |
2,4-dimethylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 460 | - |
4-methoxybenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 660 | - |
3-methylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 860 | - |
4-bromobenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 1000 | - |
4-methylbenzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
| 1100 | - |
benzaldehyde | pH 4.2, 22°C | Chamberlinius hualienensis | |
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Release 2023.1 (January 2023)
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