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Literature summary for 4.1.2.10 extracted from
- Purification and characterization of a novel (R)-mandelonitrile lyase from the fern Phlebodium aureum (1995), Plant Physiol., 109, 1231-1238.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 0.2 mM, 85% inhibition | Prunus dulcis |  |
| diethyl dicarbonate | 0.5 mM, 60% inhibition | Phlebodium aureum | |
| diethyl dicarbonate | 0.5 mM, 50% inhibition. 2 mM, 79% inhibition | Prunus dulcis | |
| diisopropyl fluorophosphate | 2 mM, 80% inhibition | Prunus dulcis | |
| iodoacetamide | 2 mM, 70% inhibition | Prunus dulcis | |
| additional information | the enzyme is not inhibited by sulfhydryl- or hydroxyl-modifying reagents | Phlebodium aureum | |
| phenylmethylsulfonyl fluoride | 2 mM, 50% inhibition | Prunus dulcis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
benzaldehyde | pH 5.3 | Phlebodium aureum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
x * 20000, SDS-PAGE | Phlebodium aureum |
| 168000 | - |
isoenzyme A and C, gel filtration | Phlebodium aureum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phlebodium aureum | - |
- |
- |
| Prunus dulcis | O24243 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phlebodium aureum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanide + benzaldehyde | - |
Phlebodium aureum | (R)-mandelonitrile | 82% yield, 99% enantiomeric excess | ? | |
| cyanide + benzaldehyde | - |
Prunus dulcis | (R)-mandelonitrile | 99% yield, 99% enantiomeric excess | ? | |
| cyanide + butanal | - |
Prunus dulcis | (2R)-2-hydroxypentanenitrile | 100% yield, 90% enantiomeric excess | ? | |
| cyanide + butanal | - |
Phlebodium aureum | (2R)-2-hydroxypentanenitrile | 42% yield, 55% enantiomeric excess | ? | |
| cyanide + pentan-2-one | - |
Prunus dulcis | (2R)-2-hydroxy-2-methylpentanenitrile | - |
? | |
| cyanide + pentan-2-one | - |
Phlebodium aureum | (2R)-2-hydroxy-2-methylpentanenitrile | 17% yield | ? | |
| cyanide + thiophene-2-carbaldehyde | - |
Prunus dulcis | (2R)-hydroxy(thiophen-2-yl)ethanenitrile | 48% yield, 87% enantiomeric excess | ? | |
| cyanide + thiophene-2-carbaldehyde | - |
Phlebodium aureum | (2R)-hydroxy(thiophen-2-yl)ethanenitrile | 87% yield, 99% enentiomeric excess | ? | |
| additional information | aliphatic carbonyls are poorly converted | Phlebodium aureum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 20000, SDS-PAGE | Phlebodium aureum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (R)-Mandelonitrile lyase | - |
Phlebodium aureum |
| (R)-Mandelonitrile lyase | - |
Prunus dulcis |
| PaMDL | - |
Prunus dulcis |
| PhaMDL | - |
Phlebodium aureum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Phlebodium aureum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | because of the base-catalyzed decomposition of mandelonitrile, pH dependence is only determined below pH 7.5. Activity increases from pH 3.5 to 6.2 and levels off between pH 6.2 and 7 for both isoforms | Phlebodium aureum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | a flavoprotein | Prunus dulcis | |
| additional information | not a flavoprotein | Phlebodium aureum |
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