BRENDA - Enzyme Database
show all sequences of 4.1.1.99

Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production

Kang, A.; George, K.W.; Wang, G.; Baidoo, E.; Keasling, J.D.; Lee, T.S.; Metab. Eng. 34, 25-35 (2016)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
isopentenol production in Escherichia coli by utilizing phosphomevalonate decarboxylase and Escherchia coli-endogenous phosphatase AphA. The enzymes bypass the isopentenyl diphosphate mevalonate pathways, have reduced energetic requirements, are further decoupled from intrinsic regulation, and are free from isopentenyl diphosphate-related toxicity. Reduced aeration rate has less impact on the bypass pathway than the original mevalonate pathway. The performance of the bypass pathway is primarily determined by the activity of phosphomevalonate decarboxylase
Haloferax volcanii
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Haloferax volcanii
Organism
Organism
UniProt
Commentary
Textmining
Haloferax volcanii
D4GXZ3
-
-
Haloferax volcanii DSM 3757
D4GXZ3
-
-
Synonyms
Synonyms
Commentary
Organism
MvaD
-
Haloferax volcanii
Application (protein specific)
Application
Commentary
Organism
synthesis
isopentenol production in Escherichia coli by utilizing phosphomevalonate decarboxylase and Escherchia coli-endogenous phosphatase AphA. The enzymes bypass the isopentenyl diphosphate mevalonate pathways, have reduced energetic requirements, are further decoupled from intrinsic regulation, and are free from isopentenyl diphosphate-related toxicity. Reduced aeration rate has less impact on the bypass pathway than the original mevalonate pathway. The performance of the bypass pathway is primarily determined by the activity of phosphomevalonate decarboxylase
Haloferax volcanii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Haloferax volcanii
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748553
Kang
Isopentenyl diphosphate (IPP) ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Metab. Eng.
34
25-35
2016
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727778
Vannice
Identification in Haloferax vo ...
Haloferax volcanii, Haloferax volcanii DSM 3757
J. Bacteriol.
196
1055-1063
2014
-
-
1
-
-
-
1
2
-
1
1
2
-
7
-
-
1
-
-
-
-
-
4
1
2
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
2
-
1
1
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-