Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl phosphate kinase as catalysts of the terminal enzyme reactions in an archaeal alternate mevalonate pathway

Vannice, J.C.; Skaff, D.A.; Keightley, A.; Addo, J.K.; Wyckoff, G.J.; Miziorko, H.M.; J. Bacteriol. 196, 1055-1063 (2014)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

6-fluoromevalonate 5'-phosphate

negligible inhibition by 6-fluoromevalonate diphosphate (a potent inhibitor of the classical mevalonate pathway), reinforcing its selectivity for monophosphorylated ligands

Haloferax volcanii

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.075

-

mevalonate 5-phosphate

pH 7.5, 30°C

Haloferax volcanii

0.289

-

ATP

pH 7.5, 30°C

Haloferax volcanii

Metals/Ions

Commentary

Organism

Structure

KCl

activity is not strongly dependent on KCl over a 0 to 4 M range

Haloferax volcanii

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + mevalonate 5-phosphate

Haloferax volcanii

the enzyme is required for the alternate mevalonate pathway in an archaea

ADP + phosphate + isopentenyl phosphate + CO2

-

-

?

ATP + mevalonate 5-phosphate

Haloferax volcanii DSM 3757

the enzyme is required for the alternate mevalonate pathway in an archaea

ADP + phosphate + isopentenyl phosphate + CO2

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

ATP + mevalonate 5-phosphate

the enzyme is required for the alternate mevalonate pathway in an archaea

727778

Haloferax volcanii

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

ATP + mevalonate 5-phosphate

very weak activity with mevalonate and even less with mevalonate 5-diphosphate

727778

Haloferax volcanii

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

ATP + mevalonate 5-phosphate

the enzyme is required for the alternate mevalonate pathway in an archaea

727778

Haloferax volcanii DSM 3757

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

ATP + mevalonate 5-phosphate

very weak activity with mevalonate and even less with mevalonate 5-diphosphate

727778

Haloferax volcanii DSM 3757

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

IC50 Value

IC50 Value Maximum

Commentary

Organism

Inhibitor

Structure

0.016

-

pH 7.5, 30°C

Haloferax volcanii

6-fluoromevalonate 5'-phosphate

IC50 Value

IC50 Value Maximum

Commentary

Organism

Inhibitor

Structure

0.016

-

pH 7.5, 30°C

Haloferax volcanii

6-fluoromevalonate 5'-phosphate

Inhibitors

Commentary

Organism

Structure

6-fluoromevalonate 5'-phosphate

negligible inhibition by 6-fluoromevalonate diphosphate (a potent inhibitor of the classical mevalonate pathway), reinforcing its selectivity for monophosphorylated ligands

Haloferax volcanii

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.075

-

mevalonate 5-phosphate

pH 7.5, 30°C

Haloferax volcanii

0.289

-

ATP

pH 7.5, 30°C

Haloferax volcanii

Metals/Ions

Commentary

Organism

Structure

KCl

activity is not strongly dependent on KCl over a 0 to 4 M range

Haloferax volcanii

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + mevalonate 5-phosphate

Haloferax volcanii

the enzyme is required for the alternate mevalonate pathway in an archaea

ADP + phosphate + isopentenyl phosphate + CO2

-

-

?

ATP + mevalonate 5-phosphate

Haloferax volcanii DSM 3757

the enzyme is required for the alternate mevalonate pathway in an archaea

ADP + phosphate + isopentenyl phosphate + CO2

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

ATP + mevalonate 5-phosphate

the enzyme is required for the alternate mevalonate pathway in an archaea

727778

Haloferax volcanii

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

ATP + mevalonate 5-phosphate

very weak activity with mevalonate and even less with mevalonate 5-diphosphate

727778

Haloferax volcanii

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

ATP + mevalonate 5-phosphate

the enzyme is required for the alternate mevalonate pathway in an archaea

727778

Haloferax volcanii DSM 3757

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

ATP + mevalonate 5-phosphate

very weak activity with mevalonate and even less with mevalonate 5-diphosphate

727778

Haloferax volcanii DSM 3757

ADP + phosphate + isopentenyl phosphate + CO2

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

748553

Kang

Isopentenyl diphosphate (IPP) ...

Haloferax volcanii, Haloferax volcanii DSM 3757

Metab. Eng.

34

25-35

2016

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727778

Vannice

Identification in Haloferax vo ...

Haloferax volcanii, Haloferax volcanii DSM 3757

J. Bacteriol.

196

1055-1063

2014

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4

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-

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1

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1

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1

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1

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1

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