BRENDA - Enzyme Database
show all sequences of 4.1.1.99

Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl phosphate kinase as catalysts of the terminal enzyme reactions in an archaeal alternate mevalonate pathway

Vannice, J.C.; Skaff, D.A.; Keightley, A.; Addo, J.K.; Wyckoff, G.J.; Miziorko, H.M.; J. Bacteriol. 196, 1055-1063 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Haloferax volcanii H1209
Haloferax volcanii
Inhibitors
Inhibitors
Commentary
Organism
Structure
6-fluoromevalonate 5'-phosphate
negligible inhibition by 6-fluoromevalonate diphosphate (a potent inhibitor of the classical mevalonate pathway), reinforcing its selectivity for monophosphorylated ligands
Haloferax volcanii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.075
-
mevalonate 5-phosphate
pH 7.5, 30°C
Haloferax volcanii
0.289
-
ATP
pH 7.5, 30°C
Haloferax volcanii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
activity is not strongly dependent on KCl over a 0 to 4 M range
Haloferax volcanii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37328
-
x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry
Haloferax volcanii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + mevalonate 5-phosphate
Haloferax volcanii
the enzyme is required for the alternate mevalonate pathway in an archaea
ADP + phosphate + isopentenyl phosphate + CO2
-
-
?
ATP + mevalonate 5-phosphate
Haloferax volcanii DSM 3757
the enzyme is required for the alternate mevalonate pathway in an archaea
ADP + phosphate + isopentenyl phosphate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Haloferax volcanii
D4GXZ3
-
-
Haloferax volcanii DSM 3757
D4GXZ3
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Haloferax volcanii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + mevalonate 5-phosphate
the enzyme is required for the alternate mevalonate pathway in an archaea
727778
Haloferax volcanii
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
ATP + mevalonate 5-phosphate
very weak activity with mevalonate and even less with mevalonate 5-diphosphate
727778
Haloferax volcanii
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
ATP + mevalonate 5-phosphate
the enzyme is required for the alternate mevalonate pathway in an archaea
727778
Haloferax volcanii DSM 3757
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
ATP + mevalonate 5-phosphate
very weak activity with mevalonate and even less with mevalonate 5-diphosphate
727778
Haloferax volcanii DSM 3757
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry
Haloferax volcanii
Synonyms
Synonyms
Commentary
Organism
HVO_1412
-
Haloferax volcanii
PMD
-
Haloferax volcanii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Haloferax volcanii
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.016
-
pH 7.5, 30°C
Haloferax volcanii
6-fluoromevalonate 5'-phosphate
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Haloferax volcanii H1209
Haloferax volcanii
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.016
-
pH 7.5, 30°C
Haloferax volcanii
6-fluoromevalonate 5'-phosphate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
6-fluoromevalonate 5'-phosphate
negligible inhibition by 6-fluoromevalonate diphosphate (a potent inhibitor of the classical mevalonate pathway), reinforcing its selectivity for monophosphorylated ligands
Haloferax volcanii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.075
-
mevalonate 5-phosphate
pH 7.5, 30°C
Haloferax volcanii
0.289
-
ATP
pH 7.5, 30°C
Haloferax volcanii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
activity is not strongly dependent on KCl over a 0 to 4 M range
Haloferax volcanii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37328
-
x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry
Haloferax volcanii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + mevalonate 5-phosphate
Haloferax volcanii
the enzyme is required for the alternate mevalonate pathway in an archaea
ADP + phosphate + isopentenyl phosphate + CO2
-
-
?
ATP + mevalonate 5-phosphate
Haloferax volcanii DSM 3757
the enzyme is required for the alternate mevalonate pathway in an archaea
ADP + phosphate + isopentenyl phosphate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Haloferax volcanii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + mevalonate 5-phosphate
the enzyme is required for the alternate mevalonate pathway in an archaea
727778
Haloferax volcanii
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
ATP + mevalonate 5-phosphate
very weak activity with mevalonate and even less with mevalonate 5-diphosphate
727778
Haloferax volcanii
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
ATP + mevalonate 5-phosphate
the enzyme is required for the alternate mevalonate pathway in an archaea
727778
Haloferax volcanii DSM 3757
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
ATP + mevalonate 5-phosphate
very weak activity with mevalonate and even less with mevalonate 5-diphosphate
727778
Haloferax volcanii DSM 3757
ADP + phosphate + isopentenyl phosphate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry
Haloferax volcanii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Haloferax volcanii
Other publictions for EC 4.1.1.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748553
Kang
Isopentenyl diphosphate (IPP) ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Metab. Eng.
34
25-35
2016
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1
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5
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1
-
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-
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1
1
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-
-
-
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-
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-
727778
Vannice
Identification in Haloferax vo ...
Haloferax volcanii, Haloferax volcanii DSM 3757
J. Bacteriol.
196
1055-1063
2014
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1
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1
2
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1
1
2
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7
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1
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-
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4
1
2
-
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1
-
-
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1
-
-
1
-
-
-
-
1
1
-
2
-
1
1
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
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-
-
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