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Literature summary for 4.1.1.95 extracted from
- Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway (2005), Chem. Biol., 12, 665-675.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Niallia circulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47800 | - |
2 * 47800, calculated from amino acid sequence | Niallia circulans |
| 49846 | - |
2 * 49846, LC-electrospray ionization mass spectrometry | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
- |
- |
| Niallia circulans NR3312 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA affinity column chromatography | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diaminopimelate | - |
Niallia circulans | ? | - |
? |  |
| diaminopimelate | - |
Niallia circulans NR3312 | ? | - |
? | |
| gamma-L-Glu-[S-BtrI acyl carrier protein] | - |
Niallia circulans | 4-aminobutanoyl-[S-BtrI acyl carrier protein] + CO2 | - |
? | |
| gamma-L-Glu-[S-BtrI acyl carrier protein] | - |
Niallia circulans NR3312 | 4-aminobutanoyl-[S-BtrI acyl carrier protein] + CO2 | - |
? | |
| L-arginine | - |
Niallia circulans | ? | - |
? | |
| L-lysine | - |
Niallia circulans | ? | - |
? | |
| L-lysine | - |
Niallia circulans NR3312 | ? | - |
? | |
| L-ornithine | - |
Niallia circulans | ? | - |
? | |
| L-ornithine | - |
Niallia circulans NR3312 | ? | - |
? | |
| additional information | the enzyme is not able to decarboxylate L-glutamate or any D-amino acids | Niallia circulans | ? | - |
? | |
| additional information | the enzyme is not able to decarboxylate L-glutamate or any D-amino acids | Niallia circulans NR3312 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 47800, calculated from amino acid sequence | Niallia circulans |
| homodimer | 2 * 49846, LC-electrospray ionization mass spectrometry | Niallia circulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| btrK | - |
Niallia circulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Niallia circulans | |
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Release 2023.1 (January 2023)
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