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Literature summary for 4.1.1.93 extracted from

  • Omura, H.; Wieser, M.; Nagasawa, T.
    Pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910, an organic-acid-requiring enzyme (1998), Eur. J. Biochem., 253, 480-484.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
Butyrate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
formate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
pimelate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
propionate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as an carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
3-methyl-2-benzothiazolinonehydrazone 1 mM, 26% inhibition Priestia megaterium
5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 40% inhibition Priestia megaterium
AgNO3 1 mM, complete inhibition Priestia megaterium
CuCl2 1 mM, 97% inhibition Priestia megaterium
cysteamine 1 mM, 25% inhibition Priestia megaterium
HgCl2 1 mM, complete inhibition Priestia megaterium
KCN 1 mM, 68% inhibition Priestia megaterium
NEM 1 mM, 15% inhibition Priestia megaterium
p-chloromercuribenzoate 1 mM, 75% inhibition Priestia megaterium
phenylhydrazine 1 mM, 84% inhibition Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, SDS-PAGE Priestia megaterium
98000
-
gel filtration Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium PYR2910
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Priestia megaterium

Storage Stability

Storage Stability Organism
-20°C, with dithiothreitol and glycerol, stable for 6 months Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium pyrrole + CO2
-
?
pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium PYR2910 pyrrole + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay, decarboxylation Priestia megaterium
45
-
-
Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7.0, stable below Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Priestia megaterium
7
-
assay, decarboxylation Priestia megaterium

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 pH 4.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable Priestia megaterium