acetate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

Butyrate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

formate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

pimelate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

propionate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as an carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

3-methyl-2-benzothiazolinonehydrazone

1 mM, 26% inhibition

Priestia megaterium

5,5'-dithiobis(2-nitrobenzoic acid)

1 mM, 40% inhibition

Priestia megaterium

AgNO3

1 mM, complete inhibition

Priestia megaterium

CuCl2

1 mM, 97% inhibition

Priestia megaterium

cysteamine

1 mM, 25% inhibition

Priestia megaterium

HgCl2

1 mM, complete inhibition

Priestia megaterium

KCN

1 mM, 68% inhibition

Priestia megaterium

NEM

1 mM, 15% inhibition

Priestia megaterium

p-chloromercuribenzoate

1 mM, 75% inhibition

Priestia megaterium

phenylhydrazine

1 mM, 84% inhibition

Priestia megaterium

52000

2 * 52000, SDS-PAGE

Priestia megaterium

98000

gel filtration

Priestia megaterium

Priestia megaterium

Priestia megaterium PYR2910

Priestia megaterium

-20°C, with dithiothreitol and glycerol, stable for 6 months

Priestia megaterium

pyrrole-2-carboxylate

the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide

711887

Priestia megaterium

pyrrole + CO2

?

pyrrole-2-carboxylate

the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide

711887

Priestia megaterium PYR2910

pyrrole + CO2

?

dimer

2 * 52000, SDS-PAGE

Priestia megaterium

30

assay, decarboxylation

Priestia megaterium

45

Priestia megaterium

50

pH 7.0, stable below

Priestia megaterium

6.5

Priestia megaterium

7

assay, decarboxylation

Priestia megaterium

4

8.5

pH 4.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity

Priestia megaterium

6

9

stable

Priestia megaterium

acetate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

Butyrate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

formate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

pimelate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

propionate

if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as an carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators

Priestia megaterium

3-methyl-2-benzothiazolinonehydrazone

1 mM, 26% inhibition

Priestia megaterium

5,5'-dithiobis(2-nitrobenzoic acid)

1 mM, 40% inhibition

Priestia megaterium

AgNO3

1 mM, complete inhibition

Priestia megaterium

CuCl2

1 mM, 97% inhibition

Priestia megaterium

cysteamine

1 mM, 25% inhibition

Priestia megaterium

HgCl2

1 mM, complete inhibition

Priestia megaterium

KCN

1 mM, 68% inhibition

Priestia megaterium

NEM

1 mM, 15% inhibition

Priestia megaterium

p-chloromercuribenzoate

1 mM, 75% inhibition

Priestia megaterium

phenylhydrazine

1 mM, 84% inhibition

Priestia megaterium

52000

2 * 52000, SDS-PAGE

Priestia megaterium

98000

gel filtration

Priestia megaterium

Priestia megaterium

-20°C, with dithiothreitol and glycerol, stable for 6 months

Priestia megaterium

pyrrole-2-carboxylate

the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide

711887

Priestia megaterium

pyrrole + CO2

?

pyrrole-2-carboxylate

the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide

711887

Priestia megaterium PYR2910

pyrrole + CO2

?

dimer

2 * 52000, SDS-PAGE

Priestia megaterium

30

assay, decarboxylation

Priestia megaterium

45

Priestia megaterium

50

pH 7.0, stable below

Priestia megaterium

6.5

Priestia megaterium

7

assay, decarboxylation

Priestia megaterium

4

8.5

pH 4.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity

Priestia megaterium

6

9

stable

Priestia megaterium

711888

Wieser

Carbon dioxide fixation by rev ...

Eur. J. Biochem.

713527

Wieser

Microbial synthesis of pyrrole ...

Tetrahedron Lett.