BRENDA - Enzyme Database
show all sequences of 4.1.1.93

Pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910, an organic-acid-requiring enzyme

Omura, H.; Wieser, M.; Nagasawa, T.; Eur. J. Biochem. 253, 480-484 (1998) View publication on PubMed

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
acetate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
Butyrate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
formate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
pimelate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
propionate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as an carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-methyl-2-benzothiazolinonehydrazone
1 mM, 26% inhibition
Priestia megaterium
5,5'-dithiobis(2-nitrobenzoic acid)
1 mM, 40% inhibition
Priestia megaterium
AgNO3
1 mM, complete inhibition
Priestia megaterium
CuCl2
1 mM, 97% inhibition
Priestia megaterium
cysteamine
1 mM, 25% inhibition
Priestia megaterium
HgCl2
1 mM, complete inhibition
Priestia megaterium
KCN
1 mM, 68% inhibition
Priestia megaterium
NEM
1 mM, 15% inhibition
Priestia megaterium
p-chloromercuribenzoate
1 mM, 75% inhibition
Priestia megaterium
phenylhydrazine
1 mM, 84% inhibition
Priestia megaterium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, SDS-PAGE
Priestia megaterium
98000
-
gel filtration
Priestia megaterium
Organism
Organism
UniProt
Commentary
Textmining
Priestia megaterium
-
-
-
Priestia megaterium PYR2910
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Priestia megaterium
Storage Stability
Storage Stability
Organism
-20°C, with dithiothreitol and glycerol, stable for 6 months
Priestia megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
pyrrole-2-carboxylate
the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide
711887
Priestia megaterium
pyrrole + CO2
-
-
-
?
pyrrole-2-carboxylate
the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide
711887
Priestia megaterium PYR2910
pyrrole + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 52000, SDS-PAGE
Priestia megaterium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay, decarboxylation
Priestia megaterium
45
-
-
Priestia megaterium
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 7.0, stable below
Priestia megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Priestia megaterium
7
-
assay, decarboxylation
Priestia megaterium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
8.5
pH 4.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity
Priestia megaterium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
stable
Priestia megaterium
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
acetate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
Butyrate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
formate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
pimelate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
propionate
if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as an carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators
Priestia megaterium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-methyl-2-benzothiazolinonehydrazone
1 mM, 26% inhibition
Priestia megaterium
5,5'-dithiobis(2-nitrobenzoic acid)
1 mM, 40% inhibition
Priestia megaterium
AgNO3
1 mM, complete inhibition
Priestia megaterium
CuCl2
1 mM, 97% inhibition
Priestia megaterium
cysteamine
1 mM, 25% inhibition
Priestia megaterium
HgCl2
1 mM, complete inhibition
Priestia megaterium
KCN
1 mM, 68% inhibition
Priestia megaterium
NEM
1 mM, 15% inhibition
Priestia megaterium
p-chloromercuribenzoate
1 mM, 75% inhibition
Priestia megaterium
phenylhydrazine
1 mM, 84% inhibition
Priestia megaterium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, SDS-PAGE
Priestia megaterium
98000
-
gel filtration
Priestia megaterium
Purification (Commentary) (protein specific)
Commentary
Organism
-
Priestia megaterium
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, with dithiothreitol and glycerol, stable for 6 months
Priestia megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
pyrrole-2-carboxylate
the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide
711887
Priestia megaterium
pyrrole + CO2
-
-
-
?
pyrrole-2-carboxylate
the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide
711887
Priestia megaterium PYR2910
pyrrole + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 52000, SDS-PAGE
Priestia megaterium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay, decarboxylation
Priestia megaterium
45
-
-
Priestia megaterium
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 7.0, stable below
Priestia megaterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Priestia megaterium
7
-
assay, decarboxylation
Priestia megaterium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
8.5
pH 4.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity
Priestia megaterium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
stable
Priestia megaterium
Other publictions for EC 4.1.1.93
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711887
Omura
Pyrrole-2-carboxylate decarbox ...
Priestia megaterium, Priestia megaterium PYR2910
Eur. J. Biochem.
253
480-484
1998
5
-
-
-
-
-
10
-
-
-
2
-
-
7
-
-
1
-
-
-
-
1
2
1
-
2
-
1
-
2
1
1
-
-
-
-
5
-
-
-
-
-
-
-
10
-
-
-
-
2
-
-
-
-
1
-
-
-
1
2
1
2
-
1
-
2
1
1
-
-
-
-
-
-
-
711888
Wieser
Carbon dioxide fixation by rev ...
Priestia megaterium, Priestia megaterium PYR2910
Eur. J. Biochem.
257
495-499
1998
2
-
-
-
-
-
1
2
-
1
-
-
-
7
-
-
-
2
-
-
-
-
6
-
-
2
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
6
-
2
-
-
-
2
-
-
-
-
1
1
-
-
-
713527
Wieser
-
Microbial synthesis of pyrrole ...
Priestia megaterium, Priestia megaterium PYR2910
Tetrahedron Lett.
39
4309-4310
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
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