Activating Compound | Comment | Organism | Structure |
---|---|---|---|
benzoylphenylalanine | Bpa, the four Bpa peptides enhance gamma-carboxylation by 1.5-2.3fold, and the rate enhancement profiles are very similar to that of proFIX19, showing that these propeptides are recognized by the carboxylase | Bos taurus | |
proFIX19 | enhances gamma-carboxylation of the synthetic FLEEL peptide by 2.2-2.3fold | Bos taurus | |
proPT18 | enhances gamma-carboxylation of the synthetic FLEEL peptide by 2.2-2.3fold | Bos taurus |
Cloned (Comment) | Organism |
---|---|
His6-tagged bovine liver carboxylase (His6-carboxylase) is produced in insect cells using a baculovirus expression system | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
proFIX19-16BPA propeptide | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values measured by hyperbolic weighted least-squares analysis | Bos taurus | |
1.5 | - |
FLEEL | proPTl8 propeptide | Bos taurus | |
1.6 | - |
FLEEL | proFIX19 propeptide | Bos taurus | |
1.8 | - |
FLEEL | proFIX 19-6BPA propeptide | Bos taurus | |
1.9 | - |
FLEEL | proFIX19-16BPA propeptide | Bos taurus | |
2 | - |
FLEEL | proFIX19-13BPA propeptide | Bos taurus | |
2 | - |
FLEEL | proFIX19-7BPApropeptide | Bos taurus | |
9 | - |
FLEEL | none propeptide | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q07175 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FLEEL + CO2 + O2 + vitamin KH2 | - |
Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
additional information | vitamin K carboxylase specifically interacts with the propeptide region of the precursor forms of vitamin K dependent proteins | Bos taurus | ? | - |
? | |
N-(bromoacetyl)-FLEELY + CO2 + O2 + vitamin KH2 | - |
Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
ProFIX 19-6BPA + CO2 + O2 + FLEEL + vitamin KH2 | TVFLDHENANKIBNRPKR | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
ProFIX 19-7BPA + CO2 + O2 + FLEEL + vitamin KH2 | TVFLDHENfiNKBLNRPKR | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
proFIX19 + CO2 + O2 + FLEEL + vitamin KH2 | TVFLDHENANKILNRPKRY | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
ProFIX19-13BPA + CO2 + O2 + FLEEL + vitamin KH2 | TVFLDBENWKILNRPKRY | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
ProFIX19-16BPA + CO2 + O2 + FLEEL + vitamin KH2 | TVBLDHENANKILNRPKRY | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
proPT28 + CO2 + O2 + FLEEL + vitamin KH2 | HVFLAPQQARSLLQRVRRANTFLEEVRK | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
proPTl8 + CO2 + O2 + FLEEL + vitamin KH2 | HVFLAPQQARSLLQRVRR | Bos taurus | ? + vitamin K epoxide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
glutamate carboxylase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0174 | - |
proFIX19-16BPA propeptide | competitive inhibition experiments using prom28 substrate | Bos taurus |