Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K210M | completely inactive | Rattus norvegicus |
K308M | retains 60% of the wild type enzyme activity | Rattus norvegicus |
K388M | retains 60% of the wild type enzyme activity | Rattus norvegicus |
General Stability | Organism |
---|---|
acetylated MCD activity reveals a time-dependent loss of specific activity, whereby 70% is lost by 30 min | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetic anhydride | 0.01 mg MCD is incubated with the 3fold excess of acetic anhydride leading to 70% loss of activity after 30 min | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
Sephadex G-25 gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme is phosphorylated at seven seryl/threonyl residues, and is acetylated at 11 lysyl residues | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose 4 Fast Flow column chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Malonyl-CoA | - |
Rattus norvegicus | Acetyl-CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCD | - |
Rattus norvegicus |