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Literature summary for 4.1.1.88 extracted from

  • Koo, J.H.; Kim, Y.S.
    Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus (1999), Eur. J. Biochem., 266, 683-690.
    View publication on PubMed
Search for more literature for 4.1.1.88

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 malonate apo-enzyme in presence of acetyl-CoA Acinetobacter calcoaceticus
0.16
-
 malonate subunit beta in presence of acetyl-CoA Acinetobacter calcoaceticus
0.16
-
 malonate subunits beta/gamma in presence of acetyl-CoA Acinetobacter calcoaceticus
3.23
-
 malonate apo-enzyme in presence of acetyl-CoA Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonate + H+
-
 Acinetobacter calcoaceticus acetate + CO2
-
 ?
malonate + H+
-
 Acinetobacter calcoaceticus MdcH acetate + CO2
-
 ?

Subunits

Subunits Comment Organism
More enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
additional information the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA Acinetobacter calcoaceticus