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Literature summary for 4.1.1.88 extracted from

  • Schmid, M.; Berg, M.; Hilbi, H.; Dimroth, P.
    Malonate decarboxylase of Klebsiella pneumoniae catalyses the turnover of acetyl and malonyl thioester residues on a coenzyme-A-like prosthetic group (1996), Eur. J. Biochem., 237, 221-228.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dithioerythritol formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme Klebsiella pneumoniae
hydroxylamine formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
30000
-
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
65000
-
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae
142000
-
gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2] malonate decarboxylation proceeds in two steps: the acetyl residue on the acyl carrier protein is first replaced by a malonyl residue which subsequently undergoes decarboxylation thereby regenerating the acetyl-S-acyl carrier protein Klebsiella pneumoniae

Renatured (Commentary)

Renatured (Comment) Organism
reacylation of the catalytically inactive SH-enzyme to form the catalytically active acetyl-S-enzyme can be achieved with acetic anhydride or more efficiently with malonyl-CoA Klebsiella pneumoniae

Subunits

Subunits Comment Organism
More presence of two forms of the enzyme: a catalytically inactive SH-enzyme and the catalytically active acetyl-S-enzyme which is formed by post-translational acetylation of the SH-enzyme with ATP, acetate and a specific ligase. the delta subunit is the acyl carrier protein of the enzyme complex Klebsiella pneumoniae
tetramer 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE Klebsiella pneumoniae