Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme | Klebsiella pneumoniae | |
hydroxylamine | formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE | Klebsiella pneumoniae |
30000 | - |
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE | Klebsiella pneumoniae |
65000 | - |
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE | Klebsiella pneumoniae |
142000 | - |
gel filtration | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a malonyl-[acyl-carrier protein] = an acetyl-[acyl-carrier protein] + CO2 | malonate decarboxylation proceeds in two steps: the acetyl residue on the acyl carrier protein is first replaced by a malonyl residue which subsequently undergoes decarboxylation thereby regenerating the acetyl-S-acyl carrier protein | Klebsiella pneumoniae |
Renatured (Comment) | Organism |
---|---|
reacylation of the catalytically inactive SH-enzyme to form the catalytically active acetyl-S-enzyme can be achieved with acetic anhydride or more efficiently with malonyl-CoA | Klebsiella pneumoniae |
Subunits | Comment | Organism |
---|---|---|
More | presence of two forms of the enzyme: a catalytically inactive SH-enzyme and the catalytically active acetyl-S-enzyme which is formed by post-translational acetylation of the SH-enzyme with ATP, acetate and a specific ligase. the delta subunit is the acyl carrier protein of the enzyme complex | Klebsiella pneumoniae |
tetramer | 1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the binding site for the acyl residues on the acyl carrier protein is 2'-(5''-phosphoribosyl)-3'-dephospho-CoA | Klebsiella pneumoniae |