Any feedback?
Literature summary for 4.1.1.86 extracted from
- Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii (1997), J. Bacteriol., 179, 5118-5125.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene dat, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain HB101 | Acinetobacter baumannii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47423 | - |
x * 47423, DNA sequence calculation | Acinetobacter baumannii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2,4-diaminobutanoate | Acinetobacter baumannii | - |
propane-1,3-diamine + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter baumannii | P56744 | strain ATCC 19606, gene dat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain HB101 to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and hydroxylapatite chromatography | Acinetobacter baumannii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
DABA AT activity compared to other Acinetobacter strains | Acinetobacter baumannii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate | - |
Acinetobacter baumannii | propylamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Acinetobacter baumannii | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate + 2-oxoglutarate | the enzyme is highly specific for 2-oxoglutarate | Acinetobacter baumannii | L-aspartic beta-semialdehyde + L-glutamic acid | - |
r | |
| L-lysine | - |
Acinetobacter baumannii | 1,5-diaminopentane + CO2 | - |
? | |
| L-ornithine | low activity | Acinetobacter baumannii | 1,4-diaminobutane + CO2 | - |
? | |
| additional information | substrate specificity of the purified recombinant enzyme, L-2,3-diaminopropionic acid is a poor substrate | Acinetobacter baumannii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47423, DNA sequence calculation | Acinetobacter baumannii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DABA AT | - |
Acinetobacter baumannii |
| L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase | - |
Acinetobacter baumannii |
| More | the enzyme belongs to the subgroup II of the aminotransferases | Acinetobacter baumannii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Acinetobacter baumannii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Acinetobacter baumannii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Acinetobacter baumannii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
