Cloned (Comment) | Organism |
---|---|
gene sgbH, expression in strain BL21(DE3) as N-terminally His10-tagged protein | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ | Escherichia coli K-12 | SgaH/UlaD and SgbH | L-xylulose 5-phosphate + CO2 | - |
? | |
additional information | Escherichia coli K-12 | enzyme catalyzes one step in the L-ascorbate utilization pathway, L-ascorbate is converted to L-xylulose, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
gene ulaD or sgaH encoded in the yia-sga operon, and gene sgbH encoded in the yia-sgb operon | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His10-tagged SgbH from strain BL21(DE3) to homogeneity, the His-tag is removed by thrombin | Escherichia coli K-12 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ | SgaH/UlaD and SgbH | Escherichia coli K-12 | L-xylulose 5-phosphate + CO2 | - |
? | |
additional information | enzyme catalyzes one step in the L-ascorbate utilization pathway, L-ascorbate is converted to L-xylulose, overview | Escherichia coli K-12 | ? | - |
? | |
additional information | enzymes SgaH/UlaD and SgbH are not active with 3-dehydro-L-gulonate | Escherichia coli K-12 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-keto-L-gulonate 6-phosphate decarboxylase | - |
Escherichia coli K-12 |
SgaH | - |
Escherichia coli K-12 |
SgbH | - |
Escherichia coli K-12 |
UlaD | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
3-dehydro-L-gulonate 6-phosphate | SgaH, pH 7.5, 25°C | Escherichia coli K-12 | |
64 | - |
3-dehydro-L-gulonate 6-phosphate | SgbH, pH 7.5, 25°C | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli K-12 |