Cloned (Comment) | Organism |
---|---|
gene ulaD, expression as His-tagged protein in strain BL21(DE3), and as selenomethionine-labeled enzyme | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled enzymes, free or complexed with inhibitor L-gulonate 6-phosphate, 15 mg/ml protein in 50 mM HEPES, pH 7.5, 5 mM MgCl2, 100 mM NaCl, room temperature, micro-batch method, 0.01 ml protein solution mixed with equal volume of crystallization solution containing 18% monomethyl PEG 5000, 50 mM NaH2PO4, 50 mM K2HPO4, and 50 mM Bis-Tris propane, pH 7.0, with or without 20 mM inhibitor L-gulonate 6-phosphate, crystals appear a few days after microseeding, growing for 1 week, X-ray diffraction structure determination and analysis at 2.0 A resolution | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-gulonate 6-phosphate | binding structure involving Glu33 and Asp62 and Mg2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, binding structure involving Glu33 and Asp62, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ | Escherichia coli | - |
L-xylulose 5-phosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39304 | gene ulaD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged UlaD from strain BL21(DE3), the His-tag is removed by thrombin | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ = L-xylulose 5-phosphate + CO2 | formation and stabilization of an enediolate anion intermediate is part of reaction mechanism, mechanism overview, the active site is located at the dimer interface, structure-function relationship, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ | - |
Escherichia coli | L-xylulose 5-phosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | (beta/alpha)8-barrel enzyme, the active site is located at the dimer interface | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
3-keto-L-gulonate 6-phosphate decarboxylase | - |
Escherichia coli |
KGPDC | - |
Escherichia coli |
More | the enzyme belongs to the orotidine 5'-monophosphate decarboxylase OMPDC suprafamily, overview | Escherichia coli |