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Literature summary for 4.1.1.83 extracted from

  • Yu, L.; Blaser, M.; Andrei, P.I.; Pierik, A.J.; Selmer, T.
    4-hydroxyphenylacetate decarboxylases: Properties of a novel subclass of glycyl radical enzyme systems (2006), Biochemistry, 45, 9584-9592.
    View publication on PubMed
Search for more literature for 4.1.1.83

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Clostridioides difficile
expressed in Escherichia coli Rosetta (DE3) pLysS cells Clostridium scatologenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.358
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes
0.388
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes
0.41
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile
0.649
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 28-32 iron atoms per native molecule Clostridioides difficile
Fe 30-32 iron atoms per native molecule Clostridium scatologenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
440000
-
 SDS-PAGE Clostridium scatologenes
445000
-
 SDS-PAGE Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-
Clostridium scatologenes
-
-
-
Clostridium scatologenes 957
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Streptactin-Macroprep column chromatography Clostridioides difficile
Streptactin-Macroprep column chromatography Clostridium scatologenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3,4-dihydroxyphenyl)acetate + H+
-
 Clostridioides difficile 4-methylcatechol + CO2
-
 ?
(3,4-dihydroxyphenyl)acetate + H+
-
 Clostridium scatologenes 4-methylcatechol + CO2
-
 ?
(3,4-dihydroxyphenyl)acetate + H+
-
 Clostridium scatologenes 957 4-methylcatechol + CO2
-
 ?
(4-hydroxyphenyl)acetate + H+ (4-hydroxyphenyl)acetate is p-cresol Clostridioides difficile 4-methylphenol + CO2
-
 ?
(4-hydroxyphenyl)acetate + H+ (4-hydroxyphenyl)acetate is p-cresol Clostridium scatologenes 4-methylphenol + CO2
-
 ?
(4-hydroxyphenyl)acetate + H+ (4-hydroxyphenyl)acetate is p-cresol Clostridium scatologenes 957 4-methylphenol + CO2
-
 ?

Subunits

Subunits Comment Organism
heterooctamer
-
 Clostridium scatologenes

Synonyms

Synonyms Comment Organism
4-hydroxyphenylacetate decarboxylase
-
 Clostridioides difficile
4-hydroxyphenylacetate decarboxylase
-
 Clostridium scatologenes
CSD
-
 Clostridium scatologenes
HPA decarboxylase
-
 Clostridioides difficile
HPA decarboxylase
-
 Clostridium scatologenes
HPD
-
 Clostridioides difficile

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
65
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile
67
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes
110
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile
135
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes