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Literature summary for 4.1.1.8 extracted from

  • Berthold, C.L.; Toyota, C.G.; Moussatche, P.; Wood, M.D.; Leeper, F.; Richards, N.G.; Lindqvist, Y.
    Crystallographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependent decarboxylases (2007), Structure, 15, 853-861.
    View publication on PubMed
Search for more literature for 4.1.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Oxalobacter formigenes

Crystallization (Commentary)

Crystallization (Comment) Organism
cocrystallization of wild-type OXC with 1 mM CoA, with a precipitating solution containing 0.5 M CaCl2, 0.1 M BisTris propane (2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl) propane-1,3-diol), pH 6.5, and 26% polyethylene glycol 550 monomethyl ether Oxalobacter formigenes

Protein Variants

Protein Variants Comment Organism
E121A 0.1% activity compared to the wild type enzyme Oxalobacter formigenes
E121Q 3.8% activity compared to the wild type enzyme Oxalobacter formigenes
E56A no activity, mutant elutes with a retention time corresponding to that of a dimer Oxalobacter formigenes
additional information deletion mutant DELTA553-565 shows almost no activity Oxalobacter formigenes
R555A 96% activity compared to the wild type enzyme Oxalobacter formigenes
S553A 15% activity compared to the wild type enzyme Oxalobacter formigenes
Y120A 0.3% activity compared to the wild type enzyme Oxalobacter formigenes
Y120F 8.2% activity compared to the wild type enzyme Oxalobacter formigenes
Y483A 1.6% activity compared to the wild type enzyme Oxalobacter formigenes
Y483F 1.9% activity compared to the wild type enzyme Oxalobacter formigenes

Inhibitors

Inhibitors Comment Organism Structure
3-deazathiamine diphosphate efficient inhibitor Oxalobacter formigenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 oxalyl-CoA mutant enzyme E121Q, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.021
-
 oxalyl-CoA mutant enzyme S553A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.0235
-
 oxalyl-CoA wild type enzyme Oxalobacter formigenes
0.024
-
 oxalyl-CoA mutant enzyme Y483A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.04
-
 oxalyl-CoA mutant enzyme Y483F, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.041
-
 oxalyl-CoA mutant enzyme E121A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.043
-
 oxalyl-CoA mutant enzyme Y120F, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.06
-
 oxalyl-CoA mutant enzyme Y120A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.066
-
 oxalyl-CoA mutant enzyme R555A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes

Organism

Organism UniProt Comment Textmining
Oxalobacter formigenes P40149
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue-sepharose column chromatography Oxalobacter formigenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxalyl-CoA
-
 Oxalobacter formigenes Formyl-CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Oxalobacter formigenes

Synonyms

Synonyms Comment Organism
Oxalyl coenzyme A decarboxylase
-
 Oxalobacter formigenes
OXC
-
 Oxalobacter formigenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 oxalyl-CoA mutant enzyme E121A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
0.26
-
 oxalyl-CoA mutant enzyme Y120A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
1.4
-
 oxalyl-CoA mutant enzyme Y483A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
1.7
-
 oxalyl-CoA mutant enzyme Y483F, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
3.3
-
 oxalyl-CoA mutant enzyme E121Q, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
7.2
-
 oxalyl-CoA mutant enzyme Y120F, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
13
-
 oxalyl-CoA mutant enzyme S553A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
85
-
 oxalyl-CoA mutant enzyme R555A, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes
88
-
 oxalyl-CoA wild type enzyme, in 25 mM sodium phosphate buffer, pH 6.5, containing 300 mM NaCl Oxalobacter formigenes

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required Oxalobacter formigenes