Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
235000 | - |
native protein with alpha6beta6-structure, determined by gel filtration | Roseovarius nubinhibens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfopyruvate | Roseovarius nubinhibens | - |
2-sulfoacetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Roseovarius nubinhibens | A3SN11 and A3SN10 | subunit alpha and beta | - |
Purification (Comment) | Organism |
---|---|
by anion exchange chromatography, hydrophobic interaction chromatography and gel filtration | Roseovarius nubinhibens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | ISM strain | Roseovarius nubinhibens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific ComDE enzyme activity in extracts from cells grown with sulfolactate is 0.9 mkat/mg protein, 0 mM Tris-HCl (pH 7.5), 1 mM thiamine diphosphate, 1 to 5 mM sulfopyruvate, and protein (0.1-1.0 mg/ml) | Roseovarius nubinhibens |
Storage Stability | Organism |
---|---|
purified ComDE is stable under an air atmosphere, it can be stored at 4°C for at least a month without loss of activity | Roseovarius nubinhibens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfopyruvate | - |
Roseovarius nubinhibens | 2-sulfoacetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 2 subunits, determined by SDS-PAGE | Roseovarius nubinhibens |
Synonyms | Comment | Organism |
---|---|---|
3-sulfopyruvate decarboxylase | - |
Roseovarius nubinhibens |
ComDE | - |
Roseovarius nubinhibens |
General Information | Comment | Organism |
---|---|---|
physiological function | ComDE is involved in sulfonate degradation | Roseovarius nubinhibens |